Key Laboratory of Dairy Science (Northeast Agricultural University), Ministry of Education, Harbin 150030, PR China.
Heilongjiang Key Laboratory for Animal Disease Control and Pharmaceutical Development, College of Veterinary Medicine, Northeast Agricultural University, Harbin 150030, PR China.
Ultrason Sonochem. 2021 Mar;71:105365. doi: 10.1016/j.ultsonch.2020.105365. Epub 2020 Oct 16.
α-lactalbumin was modified by ultrasound (US, 20 kHz, 43 ± 3.4 W/cm) pre-treatments (0, 15, 30 and 60 min) and laccase cross-linking of sonicated α-lactalbumin was used to evaluate the physical and oxidative stability of conjugated linoleic acid (CLA) emulsions. The emulsions prepared with laccase cross-linking US-α-lactalbumin (α-lactalbumin treated with US pre-treatment) and US-α-lactalbumin were scrutinized for oxidative and physical stability at room temperature for two weeks of storage. Laccase cross-linking US-α-lactalbumin (Lac-US-α-lactalbumin) revealed improved physical stability in comparison with US-α-lactalbumin, specified by droplet size, structural morphology, adsorbed protein, emulsifying properties and creaming index. SDS-PAGE analysis showed that there was formation of polymers in Lac-US-α-lactalbumin emulsion. Surface hydrophobicity of Lac-US-α-lactalbumin was higher than that of US-α-lactalbumin, and gradually enhanced with the increase of ultrasound time. More importantly, the measurements of peroxide values and conjugated dienes were used to study the oxidative stability of the CLA emulsions. The Lac-US-α-lactalbumin emulsion proved to be reducing the synthesis of fatty acid hydroperoxides and less conjugated dienes compared to the native and US-α-lactalbumin emulsions. This study revealed that the combination of US pre-treatment and laccase cross-linking might be an effective technique for the modification of CLA emulsions.
α-乳白蛋白经超声（US，20 kHz，43±3.4 W/cm）预处理（0、15、30 和 60 min）进行修饰，然后用漆酶交联超声处理的α-乳白蛋白，以评估共轭亚油酸（CLA）乳液的物理和氧化稳定性。用漆酶交联超声-α-乳白蛋白（用 US 预处理处理的α-乳白蛋白）和超声-α-乳白蛋白制备的乳液在室温下储存两周，考察其氧化和物理稳定性。与超声-α-乳白蛋白相比，漆酶交联超声-α-乳白蛋白（Lac-US-α-乳白蛋白）显示出更好的物理稳定性，表现在粒径、结构形态、吸附蛋白、乳化性能和乳状液分层指数上。SDS-PAGE 分析表明，Lac-US-α-乳白蛋白乳液中形成了聚合物。Lac-US-α-乳白蛋白的表面疏水性高于 US-α-乳白蛋白，并且随着超声时间的增加而逐渐增强。更重要的是，通过过氧化物值和共轭二烯的测量来研究 CLA 乳液的氧化稳定性。与天然和 US-α-乳白蛋白乳液相比，Lac-US-α-乳白蛋白乳液证明可以减少脂肪酸氢过氧化物的合成和较少的共轭二烯。本研究表明，US 预处理和漆酶交联的结合可能是修饰 CLA 乳液的有效技术。
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