Yamanaka T, Hirabayashi Y, Koketsu K, Higashi H, Matsumoto M
Department of Pediatrics, Yaizu Municipal Hospital, Japan.
Jpn J Exp Med. 1987 Apr;57(2):131-5.
We have developed a new method for detection and determination of ganglio-series gangliosides by TLC/enzyme-immunostaining. By using this method, even 1 pmole of the gangliosides in cerebrospinal fluid (CSF) from gangliosidoses and other neurological patients could be measured quantitatively. The accumulation of the gangliosides in CSF was demonstrated from patients with gangliosidoses, thus, the diagnosis could be confirmed antemortem.
