Hirabayashi Y, Koketsu K, Higashi H, Suzuki Y, Matsumoto M, Sugimoto M, Ogawa T
Biochim Biophys Acta. 1986 Mar 21;876(1):178-82.
An immunochemical method has been developed for sensitive detection and determination of ganglio-series gangliosides using thin-layer chromatography/enzyme-immunostaining. After chromatography of gangliosides, the plate was treated with Arthrobacter ureafaciens sialidase to remove all sialic acids from ganglio-series gangliosides. For the complete hydrolysis of gangliosides, sodium taurodeoxycholate was found to be required. The resulting asialo-glycolipids, GA2 and GA1 were reacted first with affinity-purified anti-GA2 and anti-GA1, respectively, and second with horseradish peroxidase-conjugated anti-rabbit IgG. Being highly sensitive and reproducible, it allows the characterization of gangliosides in cerebrospinal fluid which cannot be detected by classical methods.
已开发出一种免疫化学方法，用于使用薄层色谱/酶免疫染色法灵敏地检测和测定神经节系列神经节苷脂。神经节苷脂色谱分离后，用脲节杆菌唾液酸酶处理薄板，以从神经节系列神经节苷脂中去除所有唾液酸。发现需要牛磺脱氧胆酸钠来完全水解神经节苷脂。所得的去唾液酸糖脂GA2和GA1首先分别与亲和纯化的抗GA2和抗GA1反应，然后与辣根过氧化物酶偶联的抗兔IgG反应。该方法高度灵敏且可重复，能够鉴定脑脊液中经典方法无法检测到的神经节苷脂。
