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蛋白质纯化技术。

Protein Purification Technologies.

机构信息

Laboratory of Enzyme Technology, Department of Biotechnology, School of Applied Biology and Biotechnology, Agricultural University of Athens, Athens, Greece.

出版信息

Methods Mol Biol. 2021;2178:3-10. doi: 10.1007/978-1-0716-0775-6_1.

Abstract

Protein Biotechnology is an exciting and fast- growing area of research, with numerous industrial applications. The growing demand for developing efficient and rapid protein purification methods is driving research and growth in this area. Advances and progress in the techniques and methods of protein purification have been such that one can reasonably expect that any protein of a given order of stability may be purified to currently acceptable standards of homogeneity. However, protein manufacturing cost remains extremely high, with downstream processing constituting a substantial proportion of the overall cost. Understanding of the methods and optimization of the experimental conditions have become critical to the manufacturing industry in order to minimize production costs while satisfying the quality as well as all regulatory requirements. New purification processes exploiting specific, effective and robust methods and chromatographic materials are expected to guide the future of the protein purification market.

摘要

蛋白质生物技术是一个令人兴奋且快速发展的研究领域,拥有众多工业应用。对开发高效、快速的蛋白质纯化方法的需求不断增长,推动了该领域的研究和发展。蛋白质纯化技术和方法的进步如此之快,以至于人们可以合理地期望任何稳定性达到一定程度的蛋白质都可以被纯化到目前可接受的均一性标准。然而,蛋白质的制造成本仍然非常高,下游加工占总成本的很大一部分。为了在满足质量和所有监管要求的同时最大限度地降低生产成本,制造行业对方法的理解和实验条件的优化已变得至关重要。利用特定、有效和稳健的方法和色谱材料的新型纯化工艺有望引领蛋白质纯化市场的未来。

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