Institute for Biotechnology and Bioengineering (IBB), Centre for Biological and Chemical Engineering, Instituto Superior Técnico, Av. Rovisco Pais, 1049-001 Lisbon, Portugal.
J Chromatogr A. 2010 Apr 16;1217(16):2296-305. doi: 10.1016/j.chroma.2009.11.034. Epub 2009 Nov 17.
The number of biotechnology-based pharmaceuticals in the late-stage pipeline has been increasing more than ever. As a result, there is an enhanced demand for more efficient and cost-effective processes. During the last years, the upstream technology for the production of biopharmaceuticals has been considerably improved. Continuous discoveries in molecular biology and genetics, combined with new advances in media and feed development, have significantly increased the production titres. In order to keep up this gain, it is now essential to design new, as well as to improve the existing downstream processes that remain an unresolved bottleneck. This review evaluates several alternatives to the currently established platforms for the downstream processing biopharmaceuticals, with main focus on aqueous two-phase extraction.
后期管道中生物技术制药的数量一直在增加。因此，人们对更高效、更具成本效益的工艺的需求也在增加。在过去的几年中，生物制药生产的上游技术得到了极大的改进。分子生物学和遗传学的不断发现，加上媒体和饲料开发方面的新进展，极大地提高了生产产率。为了保持这一增长，现在必须设计新的方法，同时改进仍然是未解决瓶颈的现有下游工艺。本综述评估了几种替代目前用于生物制药下游处理的现有平台的方法，主要侧重于双水相萃取。
