Tecnologico de Monterrey, Escuela de Ingenieria y Ciencias, Monterrey, Mexico.
Tecnologico de Monterrey, Escuela de Medicina y Ciencias de la Salud, Monterrey, Mexico.
Methods Mol Biol. 2021;2178:65-79. doi: 10.1007/978-1-0716-0775-6_7.
The increasing interest of the biopharmaceutical industry to exploit plants as a commercially viable production system is demanding the development of new strategies to maximize product recovery. Aqueous two-phase systems (ATPSs) are a primary recovery technique that has shown great potential for the efficient extraction and purification of biological products, from organelles to proteins and low-molecular-weight compounds. The evaluation of different system parameters upon the partitioning behavior can provide the conditions that favor the concentration of contaminants and the desired target protein in opposite phases. The protocols described here provide the basic strategy to explore the use of ATPSs for the isolation and partial purification of native and recombinant proteins from plants and plant-derived extracts.
生物制药行业越来越有兴趣利用植物作为一种商业可行的生产系统，这就要求开发新的策略来最大限度地回收产品。双水相系统（ATPS）是一种主要的回收技术，它在有效提取和纯化生物产品方面显示出巨大的潜力，从细胞器到蛋白质和低分子量化合物。评估不同系统参数对分配行为的影响，可以提供有利于污染物浓缩和所需目标蛋白在相反相中的条件。这里描述的方案提供了从植物和植物提取物中分离和部分纯化天然和重组蛋白质的基本策略，以探索使用双水相系统。
