Departamento de Biotecnología e Ingeniería de Alimentos, Centro de Biotecnología-FEMSA, Tecnológico de Monterrey, Campus Monterrey, Ave. Eugenio Garza Sada 2501 Sur, Monterrey, NL, Mexico.
J Sci Food Agric. 2010 Jul;90(9):1385-92. doi: 10.1002/jsfa.3956.
The increasing interest of the biopharmaceutical industry to exploit plants as economically viable production systems is demanding the development of new downstream strategies to maximize product recovery. Aqueous two-phase systems (ATPSs) are a primary recovery technique that has shown great potential for the efficient extraction and purification of biological compounds. The present paper gives an overview of the efficient use of ATPS-based strategies for the isolation and partial purification of bioparticles from plant origin. Selected examples highlight the main advantages of this technique, i.e. scaling-up feasibility, process integration capability and biocompatibility. An overview of the recent approach of coupling ATPSs with traditional techniques to increase bioseparation process performance is discussed. A novel approach to characterization protein from plants combining ATPSs and two-dimensional electrophoresis (2-DE) is introduced as a tool for process development. In the particular case of products from plant origin, early success has demonstrated the potential application of ATPS-based strategies to address the major disadvantages of the traditional recovery and purification techniques. This literature review discloses the relevant contribution of ATPSs to facilitate the establishment of bioprocesses in the growing field of high-value products from plants.
越来越多的生物制药行业对利用植物作为经济可行的生产系统产生兴趣，这就要求开发新的下游策略，以最大限度地提高产品回收率。双水相系统（ATPS）是一种主要的回收技术，它在有效提取和纯化生物化合物方面显示出巨大的潜力。本文概述了基于 ATPS 的策略在从植物来源中分离和部分纯化生物颗粒方面的有效应用。选定的示例突出了该技术的主要优点，即扩大规模的可行性、过程集成能力和生物相容性。还讨论了最近将 ATPS 与传统技术结合以提高生物分离过程性能的方法。本文提出了一种将双水相系统与二维电泳（2-DE）相结合的方法来对植物蛋白进行分析，作为一种用于过程开发的工具。在植物来源的产品的特殊情况下，早期的成功已经证明了基于 ATPS 的策略在解决传统回收和纯化技术的主要缺点方面具有潜在的应用。本文献综述揭示了 ATPS 在促进高附加值植物产品生物工艺领域发展方面的重要贡献。
