Department of Biochemistry, Faculty of Biological Science, Tarbiat Modares University, Tehran, Iran.
Department of Biotechnology, College of Science, University of Tehran, Tehran, Iran.
Iran Biomed J. 2021 Jan;25(1):8-20. doi: 10.29252/ibj.25.1.8. Epub 2020 Sep 12.
Azo dyes are the most widely used synthetic colorants in the textile, food, pharmaceutical, cosmetic, and other industries, accounting for nearly 70% of all dyestuffs consumed. Recently, much research attention has been paid to efficient monitoring of these hazardous chemicals and their related metabolites because of their potentially harmful effect on environmental issues. In contrast to the complex and expensive instrumental procedures, the detection system based on the quantum dots (QDs) with the superior optochemical properties provides a new era in the pollution sensing and prevention.
We have developed a QD-enzyme hybrid system to probe methyl red (MR) in aqueous solutions using a fluorescence quenching procedure.
The azoreductase enzyme catalyzed the reduction of azo group in MR, which can efficiently decrease the Förster resonance energy transfer between the QDs and MR molecules. The correlation between the QDs photoluminescence recovery and MR enzymatic decolorization at the neutral phosphate buffer permitted the creation of a fluorescence quenching-based sensor. The synthesized biosensor can be used for the accurate detection of MR in a linear calibration over MR concentrations of 5-84 μM, with the limit of detection of 0.5 μM in response time of three minutes.
Our findings revealed that this fluorometric sensor has the potential to be successfully applied for monitoring a wide linear range of MR concentration with the relative standard deviation of 4% rather than the other method.
偶氮染料是纺织、食品、制药、化妆品和其他行业中使用最广泛的合成着色剂，占所用染料的近 70%。由于其对环境问题的潜在有害影响，最近人们对这些有害化学物质及其相关代谢物的有效监测给予了极大关注。与复杂且昂贵的仪器程序相比，基于具有优异光化学性质的量子点 (QD) 的检测系统为污染感应和预防提供了一个新时代。
我们开发了一种 QD-酶混合系统，通过荧光猝灭程序探测水溶液中的甲基红 (MR)。
偶氮还原酶催化 MR 中偶氮基团的还原，这可以有效地降低 QD 和 MR 分子之间的Förster 共振能量转移。在中性磷酸盐缓冲液中，QD 光致发光恢复与 MR 酶褪色之间的相关性允许创建基于荧光猝灭的传感器。该合成的生物传感器可用于在 5-84 μM 的 MR 浓度范围内进行 MR 的准确检测，响应时间为三分钟时检测限为 0.5 μM。
我们的研究结果表明，该荧光传感器具有成功应用于监测广泛线性范围内的 MR 浓度的潜力，其相对标准偏差为 4%，而不是其他方法。
