Striated Muscle Study Laboratory, Federal University of Mato Grosso do Sul (UFMS), Campo Grande, Brazil.
Internal Medicine Department, Botucatu Medical School, Sao Paulo State University (UNESP), Botucatu, Brazil.
Life Sci. 2021 Jan 1;264:118697. doi: 10.1016/j.lfs.2020.118697. Epub 2020 Oct 30.
To evaluate the influence of intermittent fasting and high-intensity interval training (HIIT) on myocardial apoptosis signaling and cardiac morphological characteristics in healthy rats.
Male Wistar rats (n = 60) were divided into four groups: sedentary control (SED-C), intermittent fasting (SED-IF), high-intensity interval training (HIIT-C), and high-intensity interval training plus intermittent fasting (HIIT-IF). SED-C and HIIT-C groups were treated daily with ad libitum chow; SED-IF and HIIT-IF received the same standard chow every other day. HIIT-C and HIIT-IF rats were submitted to an HIIT protocol five times a week for 12 weeks. At the end of the experiment, functional capacity, cardiac morphology, and expression of apoptosis signaling pathways-related proteins were analyzed.
HIIT increased cardiomyocyte cross-sectional area, collagen interstitial fraction, and the pro-apoptotic proteins AIF and caspase-3 expression, and reduced pro-apoptotic protein CYTC expression and the cleaved-to-non-cleaved PARP-1 ratio in myocardium. Intermittent fasting reduced cardiomyocyte cross-sectional area, collagen interstitial fraction, and expression of Bax, CYTC and cleaved PARP-1, and increased expression of the anti-apoptotic protein BCL-2. SMAC, ARC, and caspase-8 expression was not changed by HIIT or intermittent fasting.
HIIT promotes cardiomyocyte hypertrophy and interstitial fibrosis, and modulates the apoptosis signaling pathway in healthy rat myocardium. Intermittent fasting reduces pro-apoptotic and increases antiapoptotic signaling, besides attenuating HIIT-induced cardiomyocyte hypertrophy and myocardial interstitial fibrosis.
评估间歇性禁食和高强度间歇训练（HIIT）对健康大鼠心肌细胞凋亡信号和心脏形态特征的影响。
雄性 Wistar 大鼠（n=60）分为四组：安静对照组（SED-C）、间歇性禁食组（SED-IF）、高强度间歇训练组（HIIT-C）和高强度间歇训练加间歇性禁食组（HIIT-IF）。SED-C 和 HIIT-C 组每天给予随意进食的标准饲料；SED-IF 和 HIIT-IF 每隔一天接受相同的标准饲料。HIIT-C 和 HIIT-IF 大鼠每周接受 5 次 HIIT 方案，共 12 周。实验结束时，分析功能能力、心脏形态和凋亡信号通路相关蛋白的表达。
HIIT 增加了心肌细胞的横截面积、胶原间质分数以及促凋亡蛋白 AIF 和 caspase-3 的表达，减少了心肌细胞中线粒体中促凋亡蛋白 CYTC 的表达和 cleaved-to-non-cleaved PARP-1 的比值。间歇性禁食降低了心肌细胞的横截面积、胶原间质分数以及 Bax、CYTC 和 cleaved PARP-1 的表达，并增加了抗凋亡蛋白 BCL-2 的表达。HIIT 或间歇性禁食均未改变 SMAC、ARC 和 caspase-8 的表达。
HIIT 促进健康大鼠心肌细胞肥大和间质纤维化，并调节凋亡信号通路。间歇性禁食减少了促凋亡信号，增加了抗凋亡信号，同时减弱了 HIIT 诱导的心肌细胞肥大和心肌间质纤维化。
