Department of Food Science and Human Nutrition, University of Illinois at Urbana-Champaign, 61801, Urbana, IL, USA; PROPAC, Research and Graduate Program in Food Science, Universidad Autonoma de Queretaro, Cerro de las Campanas S/N, 76010, Queretaro, Qro, Mexico.
PROPAC, Research and Graduate Program in Food Science, Universidad Autonoma de Queretaro, Cerro de las Campanas S/N, 76010, Queretaro, Qro, Mexico.
Food Chem Toxicol. 2020 Dec;146:111835. doi: 10.1016/j.fct.2020.111835. Epub 2020 Oct 29.
Bioactive compounds from food products made from natural ingredients such as corn and common bean could target the NLRP3 inflammasome, protein scaffolds with a key role in the moderation of intestinal inflammation. This research aimed to evaluate the anti-inflammatory effect from the fermented non-digestible fraction of baked corn and common bean snack (FNDF), and its main components, on the modulation of NLRP3 inflammasome markers in vitro. For this, a THP-1 macrophage/differentiated Caco-2 cell co-culture was used as a model of intestinal inflammation. A disease control (DC) (LPS/human IFN-γ, 10 ng/mL) was compared with FNDF (40-300 μg/mL) and its pure components: gallic (38.85 μM) and butyric acids (6 μM), verbascose (0.06 μM), their mixture, and an anti-inflammatory control (tofacitinib, 5 μM). Compared to DC, FNDF (40 μg/mL) reduced the 48 h-basolateral nitrites (40-60%), IL-1β/IL-18, and TNF-α production. Additionally, it decreased the total reactive oxygen species (36.3%) and nitric oxide synthase (6.9%) activities, increasing superoxide dismutase (228.2%) activity. Compared to NLRP3 positive control, FNDF components decreased NLRP3 markers (caspase-1 activity, IL-1β, and apoptosis). These results highlight NLRP3-anti-inflammatory effects from FNDF components. This is the first report of the NLRP3 inflammasome modulation by digested food matrix components, using a co-culture approach.
来自天然成分（如玉米和普通豆）制成的食品的生物活性化合物可以靶向 NLRP3 炎性体，该蛋白支架在调节肠道炎症中起着关键作用。本研究旨在评估烘焙玉米和普通豆小吃的不可消化部分（FNDF）及其主要成分的抗炎作用，及其对体外 NLRP3 炎性体标志物的调节作用。为此，使用 THP-1 巨噬细胞/分化的 Caco-2 细胞共培养物作为肠道炎症模型。将疾病对照（DC）（LPS/人 IFN-γ，10ng/mL）与 FNDF（40-300μg/mL）及其纯成分：没食子酸（38.85μM）和丁酸（6μM）、毛蕊花糖苷（0.06μM）、它们的混合物以及抗炎对照（托法替尼，5μM）进行比较。与 DC 相比，FNDF（40μg/mL）降低了 48 h 基底外侧亚硝酸盐（40-60%）、IL-1β/IL-18 和 TNF-α 的产生。此外，它还降低了总活性氧（36.3%）和一氧化氮合酶（6.9%）的活性，同时增加了超氧化物歧化酶（228.2%）的活性。与 NLRP3 阳性对照相比，FNDF 成分降低了 NLRP3 标志物（半胱天冬酶-1 活性、IL-1β 和细胞凋亡）。这些结果强调了 FNDF 成分对 NLRP3 的抗炎作用。这是首次使用共培养方法报道消化食物基质成分对 NLRP3 炎性体的调节作用。
