Andersen Petter Vejle, Afseth Nils Kristian, Gjerlaug-Enger Eli, Wold Jens Petter
Nofima, Osloveien 1, 1430 Ås, Norway.
Norsvin, Storhamargata 44, 2317 Hamar, Norway.
Meat Sci. 2021 Feb;172:108357. doi: 10.1016/j.meatsci.2020.108357. Epub 2020 Oct 22.
The main purpose of this study was to investigate if Raman spectra recorded at the exact same position as drip loss measurements could improve prediction of drip loss in pork. One ventral and one dorsal cylindrical plug, cut from a standardized slice from Longissimus lumborum, were used to determine drip loss by EZ-DripLoss method and to collect Raman spectra, while ultimate pH was measured at another location. Partial least squares regression models were developed using spectra from each plug individually or averaged spectra from both plugs. The best models used spectra from the ventral plug, resulting in r=0.75, root mean square error of cross-validation (RMSECV) = 1.27% and ratio of prediction to deviation (RPD) =2.0 for EZ-DripLoss and r=0.72, RMSECV = 0.05 and RPD = 2.0 for ultimate pH. Results indicate that Raman spectroscopy can be used for rough screening of drip loss and pH in pork, and that the location chosen for collection of spectra can be very important for successful predictions.
本研究的主要目的是调查在与滴水损失测量完全相同的位置记录的拉曼光谱是否能改善对猪肉滴水损失的预测。从腰大肌的标准化切片上切下一个腹侧圆柱形塞子和一个背侧圆柱形塞子，用于通过EZ - DripLoss方法测定滴水损失并收集拉曼光谱，同时在另一个位置测量最终pH值。使用每个塞子的光谱单独建立或两个塞子的平均光谱建立偏最小二乘回归模型。最佳模型使用腹侧塞子的光谱，对于EZ - DripLoss，相关系数r = 0.75，交叉验证均方根误差（RMSECV）= 1.27%，预测偏差比（RPD）= 2.0；对于最终pH值，r = 0.72，RMSECV = 0.05，RPD = 2.0。结果表明，拉曼光谱可用于猪肉滴水损失和pH值的初步筛选，并且所选的光谱采集位置对于成功预测可能非常重要。
