在脑线粒体分离过程中应该使用纳加酶吗？
Should nagarse be used during the isolation of brain mitochondria?
作者信息
Wilson E J
机构信息
Department of Clinical Neurological Sciences, University of Western Ontario, Victoria Hospital, London, Canada.
出版信息
Neurochem Res. 1987 Sep;12(9):831-4. doi: 10.1007/BF00971523.
When nagarse is used to isolate brain mitochondria, a proportion of the nagarse stays associated with the mitochondrial fraction. This results in no detrimental affect on the respiratory activities. The nagarse is active in the presence of 2.3% sodium dodecyl sulfate and when samples are prepared for sodium dodecyl sulfate-polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulfate, the nagarse degrades a substantial amount of the mitochondrial proteins.
当使用纳加酶分离脑线粒体时，一部分纳加酶会与线粒体部分结合。这对呼吸活动没有不利影响。纳加酶在2.3%十二烷基硫酸钠存在的情况下具有活性，并且当在十二烷基硫酸钠存在的情况下为十二烷基硫酸钠-聚丙烯酰胺凝胶电泳制备样品时，纳加酶会降解大量的线粒体蛋白质。
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