Graduate School of Science and Engineering, Saitama University, 255 Shimo-Okubo Sakura, Saitama, 338-8570, Japan.
Anal Sci. 2021 Jan 10;37(1):17-26. doi: 10.2116/analsci.20SAR18. Epub 2020 Oct 30.
DNA aptamers, which are short, single-stranded DNA sequences that selectively bind to target substances (proteins, cells, small molecules, metal ions), can be acquired by means of the systematic evolution of ligands by exponential enrichment (SELEX) methodology. In the SELEX procedure, one of the keys for the effective acquisition of high-affinity and functional aptamer sequences is the separation stage to isolate target-bound DNA from unbound DNA in a randomized DNA library. In this review, various remarkable advancements in separation techniques for SELEX-based aptamer selection developed in this decade, are described and discussed, including CE-, microfluidic chip-, solid phase-, and FACS-based SELEX along with other methods.
DNA 适体是短的单链 DNA 序列，能够选择性地结合目标物质（蛋白质、细胞、小分子、金属离子），可以通过指数富集的配体系统进化（SELEX）方法获得。在 SELEX 过程中，有效获得高亲和力和功能适体序列的关键之一是分离阶段，即在随机 DNA 文库中从未结合的 DNA 中分离出与靶标结合的 DNA。在这篇综述中，描述并讨论了过去十年中基于 SELEX 的适体选择中各种显著的分离技术进展，包括 CE、微流控芯片、固相和基于 FACS 的 SELEX 以及其他方法。
