Gopinath Subash Chandra Bose
Functional Nucleic Acids Group, Institute for Biological Resources and Functions, National Institute of Advanced Industrial Science and Technology (AIST), 1-1-1 Higashi, Tsukuba, Ibaraki, 305-8566, Japan.
Anal Bioanal Chem. 2007 Jan;387(1):171-82. doi: 10.1007/s00216-006-0826-2. Epub 2006 Oct 28.
SELEX (systematic evolution of ligands by exponential enrichment) is a process that involves the progressive purification from a combinatorial library of nucleic acid ligands with a high affinity for a particular target by repeated rounds of partitioning and amplification. With the development of aptamer technology over the last decade, various modified SELEX processes have arisen that allow various aptamers to be developed against a wide variety of molecules, irrespective of the target size. In the present review, the separation methods used in such SELEX processes are reviewed.
指数富集配体系统进化技术（SELEX）是一个通过多轮分区和扩增，从对特定靶标具有高亲和力的核酸配体组合文库中逐步纯化的过程。在过去十年中，随着适体技术的发展，出现了各种改良的SELEX方法，这些方法能够针对各种分子开发出不同的适体，而不受靶标大小的限制。在本综述中，将对这些SELEX过程中使用的分离方法进行综述。
