Gifu Prefectural Research Institute for Food Sciences.
Saladcosmo Co. Ltd.
J Nutr Sci Vitaminol (Tokyo). 2020;66(5):481-487. doi: 10.3177/jnsv.66.481.
We determined the physiological effects of glucotropaeolin-rich lyophilized garden cress sprout powder (GC) administered to fasting and nonfasting mice. High-performance liquid chromatography analysis revealed glucotropaeolin (57.4±1.1 mg/g dry weight) as a major phytochemical constituent of GC. Decreasing tendency in body weight and feeding efficiency ratio were detected in the group of mice fed 0.05% (w/w) GC (GC0.05). Nonfasting mice exhibited significantly lower liver weights that were unchanged after fasting. Decreased total lipid (TL) and triglyceride (TG) levels in the liver were detected in the nonfasted GC0.01 and GC0.05 groups, but only in TLs of the fasted groups. The levels of plasma TGs and nonesterified fatty acids of the GC0.05 group, which remained unchanged during nonfasting, decreased after fasting. To determine its effects on the accumulation of lipids in the liver, the glucotropaeolin aglycone, benzyl isothiocyanate (BITC), was added to the liver-derived HepG2 human cell line cultured in a medium containing a high concentration of D-glucose (4,500 mg/L D-glucose) (HG group) or 1 mM oleic acid (SO group). Toxicity was not detected when cells were treated with as much as 5 μM BITC; however, lipid accumulation was inhibited by BITC in a concentration-dependent manner in the HG groups. The same effect was observed when 2 μM BITC was added to the diet of the SO groups. These results suggest that moderate levels of GC or BITC are useful for reducing liver and plasma TGs.
我们测定了富含硫葡萄糖苷的冻干荠蓝芽粉（GC）对禁食和不禁食的小鼠的生理效应。高效液相色谱分析表明，硫葡萄糖苷（57.4±1.1 mg/g 干重）是 GC 的主要植物化学成分。在喂食 0.05%（w/w）GC（GC0.05）的小鼠组中，体重和摄食效率比呈下降趋势。非禁食组的小鼠肝重明显降低，禁食后无变化。非禁食 GC0.01 和 GC0.05 组的肝总脂质（TL）和甘油三酯（TG）水平降低，但仅在禁食组的 TLs 中降低。GC0.05 组的血浆 TG 和非酯化脂肪酸在非禁食时保持不变，禁食后降低。为了确定其对肝脏脂质积累的影响，将硫葡萄糖苷苷元，苄基异硫氰酸酯（BITC）添加到高浓度 D-葡萄糖（4,500 mg/L D-葡萄糖）（HG 组）或 1 mM 油酸（SO 组）培养的肝源性 HepG2 人细胞系中。当细胞用高达 5 μM 的 BITC 处理时，没有检测到毒性；然而，BITC 以浓度依赖的方式抑制 HG 组中的脂质积累。当向 SO 组的饮食中添加 2 μM BITC 时，也观察到相同的效果。这些结果表明，适量的 GC 或 BITC 可用于降低肝和血浆 TG。
