Gershon M D, Dreyfus C F, Pickel V M, Joh T H, Reis D J
Proc Natl Acad Sci U S A. 1977 Jul;74(7):3086-9. doi: 10.1073/pnas.74.7.3086.
A specific antibody to tryptophan hydroxylase [L-tryptophan, tetrahydropteridine:oxygen oxidoreductase (5-hydroxylating), EC 1.14.16.4] has been used to localize the enzyme immunohistochemically in neurons of the mammalian gut. The enzyme was found in perikarya of intestinal neurons of mice, rats, and guinea pigs. Neurons containing the enzyme survived for up to 3 weeks in organotypic tissue culture and were intrinsic to the gut. These neurons are probably serotonergic and are the first such neurons to be found in the peripheral nervous system.
一种针对色氨酸羟化酶[L-色氨酸，四氢生物蝶呤：氧氧化还原酶（5-羟化），EC 1.14.16.4]的特异性抗体已被用于通过免疫组织化学方法在哺乳动物肠道神经元中定位该酶。在小鼠、大鼠和豚鼠的肠道神经元胞体中发现了该酶。含有该酶的神经元在器官型组织培养中存活长达3周，且是肠道固有的。这些神经元可能是5-羟色胺能神经元，是在周围神经系统中首次发现的此类神经元。
