Eddabra Rkia, Neffa Mounsef
Higher Institute of Nurses Professions and Health Techniques of Guelmim, Guelmim, Morocco.
Laboratory of Microbial Biotechnology and Plant Protection, Faculty of Sciences, University Ibn Zohr, Agadir, Morocco.
Interdiscip Perspect Infect Dis. 2020 Oct 9;2020:5185896. doi: 10.1155/2020/5185896. eCollection 2020.
In recent years, the treatment of tuberculosis has been threatened by the increasing number of patients with drug resistance, especially rifampicin resistance, which is the most effective first-line antibiotic against .
We performed a systematic review of the literature by searching the PubMed database for studies of rifampicin-resistant (MTB) isolates from Moroccan patients, published between 2010 and 2020. The aim of this review was to quantify the frequency of the most common mutations associated with rifampicin resistance, to describe the frequency at which these mutations co-occur. Identified studies were critically appraised according to the Quality Assessment of Diagnostic Accuracy Studies-2 (QUADAS-2) tool.
6 studies met our inclusion criteria. Results show that 99.36% of MTB isolates had a single-point mutation, and the most commonly mutated codon of B gene is 531 with 70.33% of phenotypically resistant strains. However, 10.38% of MTB strains phenotypically resistant to RIF did not exhibit any mutation in the gene.
Identification of a resistance-associated mutation to rifampicin can be a good marker of drug-resistant TB, but lack of a mutation in the target sequence must be interpreted with caution.
近年来，结核病的治疗受到耐药患者数量增加的威胁，尤其是对利福平耐药，利福平是对抗……最有效的一线抗生素。
我们通过在PubMed数据库中搜索2010年至2020年间发表的关于摩洛哥患者耐利福平结核分枝杆菌（MTB）分离株的研究，对文献进行了系统综述。本综述的目的是量化与利福平耐药相关的最常见突变的频率，描述这些突变同时出现的频率。根据诊断准确性研究质量评估-2（QUADAS-2）工具对纳入的研究进行严格评估。
6项研究符合我们的纳入标准。结果显示，99.36%的MTB分离株有单点突变，B基因最常发生突变的密码子是531，70.33%的表型耐药菌株为此突变。然而，10.38%表型对利福平耐药的MTB菌株在该基因中未表现出任何突变。
鉴定利福平耐药相关突变可作为耐药结核病的良好标志物，但目标序列中缺乏突变必须谨慎解读。
