Liu Lei, Yu Ying, Peng Qiuyue, Porsborg Simone R, Nielsen Frederik M, Jørgensen Annemette, Grove Anni, Bath Chris, Hjortdal Jesper, Christiansen Ole B, Fink Trine, Zachar Vladimir
Regenerative Medicine Group, Department of Health Science and Technology, Aalborg University, Aalborg, Denmark.
Department of Neurosurgery, First Hospital of Jilin University, Changchun, China.
J Ophthalmic Vis Res. 2020 Oct 25;15(4):493-501. doi: 10.18502/jovr.v15i4.7780. eCollection 2020 Oct-Dec.
To reveal the phenotypic differences between human ocular surface stromal cells (hOSSCs) cultured from the corneal, limbal, and scleral compartments.
A comparative analysis of cultured hOSSCs derived from four unrelated donors was conducted by multichromatic flow cytometry for six distinct CD antigens, including the CD73, CD90, CD105, CD166, CD146, and CD34.
The hOSSCs, as well as the reference cells, displayed phenotypical profiles that were similar in high expression of the hallmark mesenchymal stem cell markers CD73, CD90, and CD105, and also the cancer stem cell marker CD166. Notably, there was considerable variation regarding the expression of CD34, where the highest levels were found in the corneal and scleral compartments. The multi-differentiation potential marker CD146 was also expressed highly variably, ranging from 9% to 89%, but the limbal stromal and endometrial mesenchymal stem cells significantly surpassed their counterparts within the ocular and reference groups, respectively. The use of six markers enabled investigation of 64 possible variants, however, just four variants accounted for almost 90% of all hOSSCs, with the co-expression of CD73, CD90, CD105, and CD166 and a combination of CD146 and CD34. The limbal compartment appeared unique in that it displayed greatest immunophenotype diversity and harbored the highest proportion of the CD146+CD34- pericyte-like forms, but, interestingly, the pericyte-like cells were also found in the avascular cornea.
Our findings confirm that the hOSSCs exhibit an immunophenotype consistent with that of MSCs, further highlight the phenotypical heterogeneity in stroma from distinct ocular surface compartments, and finally underscore the uniqueness of the limbal region.
揭示从角膜、角膜缘和巩膜区培养的人眼表基质细胞（hOSSCs）之间的表型差异。
通过多色流式细胞术对来自四名无关供体的培养hOSSCs进行比较分析，检测六种不同的CD抗原，包括CD73、CD90、CD105、CD166、CD146和CD34。
hOSSCs以及对照细胞显示出相似的表型特征，即标志性间充质干细胞标志物CD73、CD90和CD105以及癌症干细胞标志物CD166高表达。值得注意的是，CD34的表达存在相当大的差异，在角膜和巩膜区发现其表达水平最高。多分化潜能标志物CD146的表达也高度可变，范围从9%到89%，但角膜缘基质细胞和子宫内膜间充质干细胞分别显著超过眼内组和对照中的对应细胞。使用六种标志物能够研究64种可能的变体，然而，仅四种变体就占了所有hOSSCs的近90%，即CD73、CD90、CD105和CD166的共表达以及CD146和CDD34的组合。角膜缘区显得独特，因为它表现出最大的免疫表型多样性，并且含有最高比例的CD146+CD34-周细胞样形式，但有趣的是，在无血管的角膜中也发现了周细胞样细胞。
我们的研究结果证实hOSSCs表现出与间充质干细胞一致的免疫表型，进一步突出了不同眼表区基质中的表型异质性，最终强调了角膜缘区的独特性。
