• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

来自三个不同来源的DBA/2小鼠中，海藻酸诱导癫痫发作的表型比较研究。

A comparative study of the phenotype with kainic acid-induced seizure in DBA/2 mice from three different sources.

作者信息

Kang Kyung-Ku, Kim Young-In, Seo Min-Soo, Sung Soo-Eun, Choi Joo-Hee, Lee Sijoon, Jung Young-Suk, Cho Joon Young, Hwang Dae Youn, Park Sang-Joon, Kim Kil Soo

机构信息

Laboratory Animal Center, Daegu-Gyeongbuk Medical Innovation Foundation, Daegu, Republic of Korea.

KPC Corporation, Gwangju, 12773 Korea.

出版信息

Lab Anim Res. 2020 Oct 28;36:39. doi: 10.1186/s42826-020-00072-y. eCollection 2020.

DOI:10.1186/s42826-020-00072-y
PMID:33134158
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7594308/
Abstract

The kainic acid-induced seizure mouse model is widely used in epilepsy research. In this study, we applied kainic acid to the subcutaneous injections of three different sources of DBA/2 mice to compare and evaluate the seizure response. The three mouse sources consisted of DBA/2Kor1 (Korea FDA source), DBA/2A (USA source), and DBA/2 (Japan source), and were purchased from different vendors. To compare the responses of DBA/2 mice to kainic acid injections, we examined the survival rate, seizure phenotype scoring, and behavioral changes. We also evaluated brain lesions using histopathological analysis. Following the administration of kainic acid, almost half of the cohort survived, and the seizure phenotype displayed a moderate level of sensitivity (2 ~ 4 out of 6). In the histopathologic analysis, there was no change in morphological features, and levels of glial fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule 1 (Iba-1) increased in the kainic acid-treated groups. However, there was no difference in the neuronal nuclei (NeuN) expression level. All the data showed that the responses in the kainic acid-treated group were similar across the three strains. In conclusion, our results suggest that the three sources of DBA/2 mice (DBA/2Kor1, DBA/2A, and DBA/2B) have similar pathological responses to kainic acid-induced seizures.

摘要

海藻酸诱导的癫痫小鼠模型广泛应用于癫痫研究。在本研究中，我们将海藻酸皮下注射到三种不同来源的DBA/2小鼠体内，以比较和评估癫痫反应。这三种小鼠来源分别为DBA/2Kor1（韩国食品药品监督管理局来源）、DBA/2A（美国来源）和DBA/2（日本来源），购自不同供应商。为了比较DBA/2小鼠对海藻酸注射的反应，我们检测了存活率、癫痫表型评分和行为变化。我们还使用组织病理学分析评估了脑损伤。注射海藻酸后，几乎一半的小鼠存活下来，癫痫表型显示出中等程度的敏感性（6分制中为2至4分）。在组织病理学分析中，形态学特征没有变化，海藻酸处理组中胶质纤维酸性蛋白（GFAP）和离子钙结合衔接分子1（Iba-1）的水平升高。然而，神经元细胞核（NeuN）表达水平没有差异。所有数据表明，海藻酸处理组中三种品系的反应相似。总之，我们的结果表明，三种来源的DBA/2小鼠（DBA/2Kor1、DBA/2A和DBA/2B）对海藻酸诱导的癫痫具有相似的病理反应。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb6/7594308/d7068793d194/42826_2020_72_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb6/7594308/116b91b6f6a2/42826_2020_72_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb6/7594308/7a2d9bc96fac/42826_2020_72_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb6/7594308/19a9e70bdaf5/42826_2020_72_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb6/7594308/1e80e46f8383/42826_2020_72_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb6/7594308/1dbbd4752ce2/42826_2020_72_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb6/7594308/d7068793d194/42826_2020_72_Fig6_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb6/7594308/116b91b6f6a2/42826_2020_72_Fig1_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb6/7594308/7a2d9bc96fac/42826_2020_72_Fig2_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb6/7594308/19a9e70bdaf5/42826_2020_72_Fig3_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb6/7594308/1e80e46f8383/42826_2020_72_Fig4_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb6/7594308/1dbbd4752ce2/42826_2020_72_Fig5_HTML.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/adb6/7594308/d7068793d194/42826_2020_72_Fig6_HTML.jpg

相似文献

1
A comparative study of the phenotype with kainic acid-induced seizure in DBA/2 mice from three different sources.来自三个不同来源的DBA/2小鼠中，海藻酸诱导癫痫发作的表型比较研究。
Lab Anim Res. 2020 Oct 28;36:39. doi: 10.1186/s42826-020-00072-y. eCollection 2020.
2
Comparison of seizure phenotype and neurodegeneration induced by systemic kainic acid in inbred, outbred, and hybrid mouse strains.近亲繁殖、远亲繁殖和杂交小鼠品系中，全身性海藻酸诱导的癫痫发作表型与神经退行性变的比较。
Eur J Neurosci. 2006 Oct;24(8):2191-202. doi: 10.1111/j.1460-9568.2006.05111.x.
3
Differential susceptibility to seizures induced by systemic kainic acid treatment in mature DBA/2J and C57BL/6J mice.成熟的DBA/2J和C57BL/6J小鼠对全身注射海藻酸诱导癫痫发作的易感性差异。
Epilepsia. 1995 Mar;36(3):301-7. doi: 10.1111/j.1528-1157.1995.tb00999.x.
4
Effects of subcutaneous injections of zinc chloride on seizures induced by noise and by kainic acid.
Epilepsia. 1990 Mar-Apr;31(2):139-44. doi: 10.1111/j.1528-1167.1990.tb06298.x.
5
Neuroprotective effects of brain-derived neurotrophic factor in seizures during development.脑源性神经营养因子在发育过程中癫痫发作时的神经保护作用。
Neuroscience. 1999;91(1):293-303. doi: 10.1016/s0306-4522(98)00609-5.
6
Increased seizure susceptibility in a mouse model of neurofibromatosis type 1.神经纤维瘤病 1 型小鼠模型中癫痫易感性增加。
Epilepsy Res. 2019 Oct;156:106190. doi: 10.1016/j.eplepsyres.2019.106190. Epub 2019 Aug 13.
7
Effect of kainic acid on unit discharge in CA1 area of hippocampal slice of DBA and C57 mice.海人酸对DBA和C57小鼠海马切片CA1区单位放电的影响。
Epilepsia. 1994 Sep-Oct;35(5):915-21. doi: 10.1111/j.1528-1157.1994.tb02534.x.
8
Mouse strain differences in kainic acid sensitivity, seizure behavior, mortality, and hippocampal pathology.小鼠品系在对 kainic 酸的敏感性、癫痫发作行为、死亡率和海马病理学方面的差异。
Neuroscience. 2003;122(2):551-61. doi: 10.1016/s0306-4522(03)00562-1.
9
Cyclooxygenase-2 selective inhibitors aggravate kainic acid induced seizure and neuronal cell death in the hippocampus.环氧化酶-2选择性抑制剂会加重海藻酸诱导的癫痫发作以及海马体中的神经元细胞死亡。
Brain Res. 1999 Oct 2;843(1-2):118-29. doi: 10.1016/s0006-8993(99)01797-7.
10
Behavioral changes and expression of heat shock protein hsp-70 mRNA, brain-derived neurotrophic factor mRNA, and cyclooxygenase-2 mRNA in rat brain following seizures induced by systemic administration of kainic acid.全身注射海藻酸诱导癫痫发作后大鼠脑内的行为变化以及热休克蛋白hsp - 70 mRNA、脑源性神经营养因子mRNA和环氧化酶-2 mRNA的表达
Brain Res. 1998 Sep 7;804(2):212-23. doi: 10.1016/s0006-8993(98)00708-2.

引用本文的文献

1
Neuroprotective effects of pink lotus oil in kainic acid-induced epilepsy.粉红莲花油在红藻氨酸诱导的癫痫中的神经保护作用。
Heliyon. 2024 Sep 19;10(18):e38070. doi: 10.1016/j.heliyon.2024.e38070. eCollection 2024 Sep 30.
2
High-Throughput Screening Assay for Detecting Drug-Induced Changes in Synchronized Neuronal Oscillations and Potential Seizure Risk Based on Ca Fluorescence Measurements in Human Induced Pluripotent Stem Cell (hiPSC)-Derived Neuronal 2D and 3D Cultures.基于人诱导多能干细胞（hiPSC）衍生神经元 2D 和 3D 培养物中钙荧光测量的高通量筛选测定法，用于检测药物诱导的同步神经元振荡变化和潜在的致痫风险。
Cells. 2023 Mar 21;12(6):958. doi: 10.3390/cells12060958.

本文引用的文献

1
Behavioral tests assessing neuropsychiatric phenotypes in adolescent mice reveal strain- and sex-specific effects.行为测试评估青少年小鼠的神经精神表型，揭示了品系和性别特异性的影响。
Sci Rep. 2020 Jul 9;10(1):11263. doi: 10.1038/s41598-020-67758-0.
2
Cadmium induces iron deficiency anemia through the suppression of iron transport in the duodenum.镉通过抑制十二指肠中的铁转运来诱发缺铁性贫血。
Toxicol Lett. 2020 Oct 10;332:130-139. doi: 10.1016/j.toxlet.2020.07.005. Epub 2020 Jul 6.
3
The adenosine A1 receptor agonist WAG 994 suppresses acute kainic acid-induced status epilepticus in vivo.
腺苷 A1 受体激动剂 WAG 994 抑制体内急性海人酸诱导的癫痫持续状态。
Neuropharmacology. 2020 Oct 1;176:108213. doi: 10.1016/j.neuropharm.2020.108213. Epub 2020 Jun 29.
4
Simultaneous calcium recordings of hippocampal CA1 and primary motor cortex M1 and their relations to behavioral activities in freely moving epileptic mice.在自由活动的癫痫小鼠中同时记录海马 CA1 和初级运动皮层 M1 的钙信号，并分析其与行为活动的关系。
Exp Brain Res. 2020 Jun;238(6):1479-1488. doi: 10.1007/s00221-020-05815-w. Epub 2020 May 18.
5
Epileptic brain fluorescent imaging reveals apigenin can relieve the myeloperoxidase-mediated oxidative stress and inhibit ferroptosis.癫痫脑荧光成像显示芹菜素可缓解髓过氧化物酶介导的氧化应激并抑制铁死亡。
Proc Natl Acad Sci U S A. 2020 May 12;117(19):10155-10164. doi: 10.1073/pnas.1917946117. Epub 2020 Apr 23.
6
Efficacy of Aerosolized Rifaximin versus Tobramycin for Treatment of Pseudomonas aeruginosa Pneumonia in Mice.雾化利福昔明对比妥布霉素治疗铜绿假单胞菌肺炎小鼠的疗效。
Antimicrob Agents Chemother. 2019 Jun 24;63(7). doi: 10.1128/AAC.02341-18. Print 2019 Jul.
7
Susceptibility to seizure-induced sudden death in DBA/2 mice is altered by adenosine.腺苷可改变DBA/2小鼠对癫痫发作诱导的猝死的易感性。
Epilepsy Res. 2016 Aug;124:49-54. doi: 10.1016/j.eplepsyres.2016.05.007. Epub 2016 May 19.
8
Neuroprotective effects of trans-caryophyllene against kainic acid induced seizure activity and oxidative stress in mice.反式石竹烯对红藻氨酸诱导的小鼠癫痫活动和氧化应激的神经保护作用。
Neurochem Res. 2015 Jan;40(1):118-23. doi: 10.1007/s11064-014-1474-0. Epub 2014 Nov 23.
9
Genetic regulatory network analysis reveals that low density lipoprotein receptor-related protein 11 is involved in stress responses in mice.遗传调控网络分析显示，低密度脂蛋白受体相关蛋白 11 参与了小鼠的应激反应。
Psychiatry Res. 2014 Dec 30;220(3):1131-7. doi: 10.1016/j.psychres.2014.09.002. Epub 2014 Sep 16.
10
Inflammatory pathways of seizure disorders.癫痫发作障碍的炎症途径。
Trends Neurosci. 2014 Feb;37(2):55-65. doi: 10.1016/j.tins.2013.11.002. Epub 2013 Dec 16.