Kongsui Ratchaniporn, Chanmanee Teera, Promsrisuk Tichanon, Klimaschewski Lars, Sriraksa Napatr, Jittiwat Jinatta, Thongrong Sitthisak
Division of Physiology, School of Medical Sciences, University of Phayao, 56000, Thailand.
Division of Anatomy, School of Medical Sciences, University of Phayao, 56000, Thailand.
Heliyon. 2024 Sep 19;10(18):e38070. doi: 10.1016/j.heliyon.2024.e38070. eCollection 2024 Sep 30.
Excitotoxicity-induced oxidative stress results in neuronal cell death. Pink lotus essential oil (PLO) is a concentrated volatile oil from lotus blossoms widely used in traditional medicine. This study aimed to explore the possible therapeutic effects of PLO and its underlying mechanisms on kainic acid (KA)-induced oxidative stress and hippocampal cell death in a mouse model of epilepsy. Mice were treated with 100 mg/kg or 200 mg/kg PLO to ameliorate neurodegeneration and seizure-induced behavior induced by KA injection. Pre- and post-treatment of PLO increased antioxidant activities, reduced the seizure score, prevented oxidative stress by increasing GSH and CAT levels, and reduced MDA (malondialdehyde) levels after KA-induced status epilepticus. KA injection created neuronal cell death in the pyramidal layers of CA1 and CA3 subfields of the hippocampus, and affected interneurons in the hilus of the dentate gyrus. PLO treatment notably diminished KA-induced neuronal cell death in these areas through activation of the Akt signaling pathway, increasing reactive astrogliosis, and up-regulation of GDNF expression. Moreover, caspase-3 expression, and microglia activation were significantly decreased in PLO treatments. Taken together, these results suggest that PLO possesses antiepileptic, anti-apoptosic, and neuroprotective effects on KA-induced epileptogenesis indicating that PLO may serve as a dietary supplement option in the treatment of epilepsy or of other neurodegenerative disorders.
兴奋性毒性诱导的氧化应激导致神经元细胞死亡。粉莲花精油（PLO）是一种从莲花中提取的浓缩挥发油，在传统医学中广泛应用。本研究旨在探讨PLO对红藻氨酸（KA）诱导的癫痫小鼠模型氧化应激和海马细胞死亡的潜在治疗作用及其潜在机制。用100mg/kg或200mg/kg的PLO处理小鼠，以改善KA注射诱导的神经退行性变和癫痫发作行为。PLO治疗前后增加了抗氧化活性，降低了癫痫发作评分，通过提高谷胱甘肽（GSH）和过氧化氢酶（CAT）水平预防了氧化应激，并降低了KA诱导的癫痫持续状态后的丙二醛（MDA）水平。KA注射导致海马CA1和CA3亚区锥体细胞层的神经元细胞死亡，并影响齿状回门区的中间神经元。PLO治疗通过激活Akt信号通路、增加反应性星形胶质细胞增生和上调胶质细胞源性神经营养因子（GDNF）表达，显著减少了这些区域KA诱导的神经元细胞死亡。此外，PLO治疗组中半胱天冬酶-3表达和小胶质细胞激活显著降低。综上所述，这些结果表明PLO对KA诱导的癫痫发生具有抗癫痫、抗凋亡和神经保护作用，表明PLO可能作为治疗癫痫或其他神经退行性疾病的膳食补充剂选择。
