Naghian Ebrahim, Marzi Khosrowshahi Elnaz, Sohouli Esmail, Pazoki-Toroudi Hamid Reza, Sobhani-Nasab Ali, Rahimi-Nasrabadi Mehdi, Ahmadi Farhad
Chemical Injuries Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Faculty of Pharmacy, Baqiyatallah University of Medical Sciences, Tehran, Iran.
Front Chem. 2020 Sep 10;8:689. doi: 10.3389/fchem.2020.00689. eCollection 2020.
With the development of nanomaterials in electrochemical sensors, the use of nanostructures to modify the electrode surface has been shown to improve the kinetics of the electron transfer process. In this study, a sensor was developed for the electrochemical determination of Acyclovir (ACV) based on the modified carbon paste electrode (CPE) by CdO/FeO. The magnetic CdO nanoparticles characterization was studied by energy-dispersive X-ray spectroscopy (EDS) and X-ray diffraction (XRD). To study of the modified CPE surface morphology, scanning electron microscopy (SEM) was used. At the optimal conditions, a noteworthy enhancement in the electrochemical behavior of ACV was observed at the surface of the modified CPE compared to the unmodified CPE. A detection limit of 300 nM and a linear range of 1-100 μM were obtained for the quantitative monitoring of ACV at the modified CPE surface using differential pulse voltammetry (DPV) in phosphate buffer. The RSD% (relative standard deviation) of the electrode response was <4.3% indicating the development of a high precision method. Also, satisfactory results were obtained in the determination of ACV with the modified electrode in tablet, blood serum, and urine samples with a satisfactory relative recovery (RR%) in the range of 94.0-104.4%.
随着纳米材料在电化学传感器中的发展，利用纳米结构修饰电极表面已被证明可以改善电子转移过程的动力学。在本研究中，基于CdO/FeO修饰的碳糊电极（CPE）开发了一种用于阿昔洛韦（ACV）电化学测定的传感器。通过能量色散X射线光谱（EDS）和X射线衍射（XRD）对磁性CdO纳米颗粒进行了表征。为了研究修饰的CPE表面形态，使用了扫描电子显微镜（SEM）。在最佳条件下，与未修饰的CPE相比，在修饰的CPE表面观察到ACV的电化学行为有显著增强。使用差分脉冲伏安法（DPV）在磷酸盐缓冲液中对修饰的CPE表面的ACV进行定量监测时，获得了300 nM的检测限和1-100 μM的线性范围。电极响应的相对标准偏差（RSD%）<4.3%，表明开发了一种高精度方法。此外，使用修饰电极对片剂、血清和尿液样品中的ACV进行测定时，也获得了令人满意的结果，相对回收率（RR%）在94.0-104.4%范围内。
