Oladejo Tolulope Christianah, Olaniyi Oladipo Oladiti, Ayodeji Adeyemi Oluwadare, Akinyele Bamidele Juliet
Department of Microbiology, Federal University of Technology, PMB 704, Akure, Nigeria.
Department of Biochemistry, Federal University of Technology, PMB 704, Akure, Nigeria.
Heliyon. 2020 Oct 23;6(10):e05123. doi: 10.1016/j.heliyon.2020.e05123. eCollection 2020 Oct.
This study evaluates the nutritional values of African yam bean hydrolyzed with protease from and afterward assess its effect on the growth and blood parameters of albino rats. The nutritional compositions of AYB hydrolyzed with partially purified protease from were determined by standard chemical methods. The protease-hydrolyzed AYB was thereafter formulated into feeds with different inclusion levels (20, 40 and 60%), which was used to feed albino rats for 27 days. After the feeding trial, the blood of anesthetized albino rats was collected using the cardiac puncture method, and the hematological parameters were determined by standard biochemical methods. The AYB hydrolyzed with partially purified protease had the highest percentage crude protein with a value of 31.2% when compared with boiled, soaked and boiled, and raw sample with the values of 20.9, 20.9 and 19.9%, respectively. The treatment of AYB with purified protease also resulted in an increase of vitamins and some essential amino acids when compared with unhydrolyzed AYB. The group of rats fed with 60% hydrolyzed AYB had the highest percentage average weight gain of approximately 144%, while the values recorded for the groups fed with commercial feed and unhydrolyzed AYB were approximately 86 and 101%, respectively. The hematological analysis revealed that the hemoglobin (Hb) and packed cell volume (PCV) of the group fed with 40% hydrolyzed AYB of 14 g/L and 38% respectively, were significantly higher than the rats fed with commercial feed with values of 10 g/L and 32%, respectively. Thus, enzyme-hydrolyzed AYB might be a suitable alternative to animal protein with good functional properties.
本研究评估了用[具体来源]的蛋白酶水解的非洲山药豆的营养价值，并随后评估其对白化大鼠生长和血液参数的影响。用来自[具体来源]的部分纯化蛋白酶水解的非洲山药豆的营养成分通过标准化学方法测定。此后，将蛋白酶水解的非洲山药豆配制成不同添加水平（20%、40%和60%）的饲料，用于喂养白化大鼠27天。喂养试验结束后，采用心脏穿刺法采集麻醉后的白化大鼠血液，并用标准生化方法测定血液学参数。与煮熟、浸泡后煮熟和生样品相比，用部分纯化蛋白酶水解的非洲山药豆的粗蛋白百分比最高，分别为20.9%、20.9%和19.9%，其值为31.2%。与未水解的非洲山药豆相比，用纯化蛋白酶处理非洲山药豆还导致维生素和一些必需氨基酸增加。喂食60%水解非洲山药豆的大鼠组平均体重增加百分比最高，约为144%，而喂食商业饲料和未水解非洲山药豆的组记录的值分别约为86%和101%。血液学分析表明，喂食40%水解非洲山药豆的组的血红蛋白（Hb）和红细胞压积（PCV）分别为14 g/L和38%，显著高于喂食商业饲料的大鼠，其值分别为10 g/L和32%。因此，酶水解的非洲山药豆可能是具有良好功能特性的动物蛋白的合适替代品。
