Ju Tuoyu, Wang Shuwei, Wang Jiajia, Yang Fan, Song Zhengxun, Xu Hongmei, Chen Yujuan, Zhang Jingran, Wang Zuobin
International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun 130022, China.
Anal Methods. 2020 Dec 7;12(45):5458-5467. doi: 10.1039/d0ay01730b. Epub 2020 Nov 2.
Tumor-derived exosomes (exos) are closely related to the occurrence, development and treatment of tumors. However, it is not clear how the exosomes affect the physical properties, which lead to the deterioration of the target cells. In this paper, atomic force microscopy (AFM) was used to study the effects of exosomes in HCC-LM3 cells and other cells (SMMC-7721 and HL-7702). The results showed that the HCC-LM3-exos (the exosomes secreted by HCC-LM3 cells, 50 μg mL) significantly promoted the proliferation and migration of HCC-LM3 cells. HCC-LM3-exos also promoted the proliferation and migration of SMMC-7721 and HL-7702 cells at 1000 and 1500 μg mL, respectively. With an increase in time and concentration, the proliferation effect was more significant. On comparing the mechanical properties of the three types of cells (HCC-LM3, SMMC-7721 and HL-7702 cells), the degradation degree and migration ability of the cells were from high to low in the above order. In turn, the surface roughness of the cells decreased, and adhesion and elastic modulus increased. With an increase in treatment time, surface roughness increased, while adhesion and elastic modulus decreased. These suggested that the HCC-LM3-exos could change the mechanical properties of cells, leading to their deterioration, and enhance their migration and invasion ability. In this paper, the effects of exosomes were analyzed from the perspective of the physical parameters of cells, which provide a new idea to study cancer metastasis and prognosis.
肿瘤来源的外泌体（exos）与肿瘤的发生、发展及治疗密切相关。然而，尚不清楚外泌体如何影响物理性质从而导致靶细胞恶化。本文利用原子力显微镜（AFM）研究了外泌体对肝癌LM3细胞及其他细胞（SMMC - 7721和HL - 7702）的影响。结果显示，HCC - LM3 - exos（HCC - LM3细胞分泌的外泌体，50 μg/mL）显著促进了HCC - LM3细胞的增殖和迁移。HCC - LM3 - exos分别在1000 μg/mL和1500 μg/mL时也促进了SMMC - 7721和HL - 7702细胞的增殖和迁移。随着时间和浓度的增加，增殖效应更为显著。比较三种类型细胞（HCC - LM3、SMMC - 7721和HL - 7702细胞）的力学性能，细胞的降解程度和迁移能力按上述顺序从高到低。相应地，细胞的表面粗糙度降低，黏附力和弹性模量增加。随着处理时间的增加，表面粗糙度增加，而黏附力和弹性模量降低。这些表明HCC - LM3 - exos可改变细胞的力学性能，导致其恶化，并增强其迁移和侵袭能力。本文从细胞物理参数的角度分析了外泌体的作用，为研究癌症转移和预后提供了新思路。
