The First Hospital, Jilin University, Changchun, 130012, China.
International Research Centre for Nano Handling and Manufacturing of China, Changchun University of Science and Technology, Changchun, 130022, China; Ministry of Education Key Laboratory for Cross-Scale Micro and Nano Manufacturing, Changchun University of Science and Technology, Changchun, 130022, China.
Micron. 2021 Apr;143:103001. doi: 10.1016/j.micron.2020.103001. Epub 2021 Jan 12.
Previous studies reported that exosomes (Exos) secreted by tumor cells could affect the tumor cells themselves and normal cells. However, the effects of exosomes derived from tumor cells on normal cells' migration and mechanical characteristics are rarely reported. This work explores the effects of H1299 cell-derived exosomes (H1299-Exos) on the migration of BEAS-2B cells, and analyzes possible mechanical mechanisms. In the experiments, exosomes were isolated from the culture supernatants of H1299 cells by ultracentrifugation. The H1299-Exos were confirmed by scanning electron microscope (SEM) and western blotting (WB). The BEAS-2B cell migration was assessed using scratch assays. Cytoskeletal structure changes were detected by immunofluorescence. Surface morphology and mechanical properties were measured by atomic force microscopy (AFM). After incubation with H1299-Exos for 48 h, BEAS-2B cells enhanced migration ability, with increased filopodia and cytoskeletal rearrangements. The changes in the morphology and mechanical properties of the cells caused by H1299-Exos were detected using AFM, including the increase in cell length and the decrease in cell height, Young's modulus and adhesion. In short, H1299-Exos promoted the BEAS-2B cell migrations. It indicates that the morphological and mechanical properties can be used as a means to assess normal cell alterations induced by tumor cell derived-exosomes. This provides a method for studying the effects of exosomes secreted by tumor cells on normal cells and the changes in their physical properties.
先前的研究报告指出，肿瘤细胞分泌的外泌体（Exos）可以影响肿瘤细胞本身和正常细胞。然而，肿瘤细胞来源的外泌体对正常细胞迁移和力学特性的影响很少有报道。本工作探讨了 H1299 细胞来源的外泌体（H1299-Exos）对 BEAS-2B 细胞迁移的影响，并分析了可能的力学机制。在实验中，通过超速离心从 H1299 细胞的培养上清液中分离出外泌体。通过扫描电子显微镜（SEM）和 Western blot（WB）证实了 H1299-Exos 的存在。通过划痕实验评估 BEAS-2B 细胞的迁移。通过免疫荧光检测细胞骨架结构的变化。通过原子力显微镜（AFM）测量表面形态和力学性能。孵育 H1299-Exos 48 小时后，BEAS-2B 细胞迁移能力增强，丝状伪足增多，细胞骨架重排。用 AFM 检测 H1299-Exos 引起的细胞形态和力学性能的变化，包括细胞长度增加，细胞高度、杨氏模量和粘附力降低。总之，H1299-Exos 促进了 BEAS-2B 细胞的迁移。这表明形态和力学特性可以作为评估肿瘤细胞来源的外泌体诱导正常细胞改变的一种手段。这为研究肿瘤细胞分泌的外泌体对正常细胞的影响及其物理性质的变化提供了一种方法。
