Petersen H D, Lindhardt B O, Nyarango P M, Bowry T R, Chemtai A K, Krogsgaard K, Bunyasi A
Department of Infectious Diseases, Rigshospitalet, University of Copenhagen, Denmark.
Scand J Infect Dis. 1987;19(4):395-401. doi: 10.3109/00365548709021671.
In order to describe the prevalence of HIV antibodies and AIDS in West Kenya, serological tests, including ELISA, and in some cases immunoblotting, were performed on whole blood collected on filter paper from 603 Kenyans. Serum samples from 55 of these persons underwent the same examinations, and 45 were further examined by immunofluorescence and a commercial ELISA. The majority of the Kenyans examined were residents of a province in West Kenya, while the others were students from other parts of Kenya, predominantly rural areas. Male/female ratio was 62/38. Median age was 18 years (range 0-70). Five Danes with previously demonstrated HIV antibodies, and 10 Danish controls were examined for HIV antibodies in filter paper whole blood, and in serum by ELISA and immunoblotting. The tests carried out on the filter paper blood were found to be reliable. Only one of the examined Kenyans had antibodies to HIV by both ELISA and immunoblotting, representing a prevalence of 0.17% (95% confidence limits: 0.00-0.93%). This low prevalence is not in accord with results previously presented from rural districts in Kenya.
为描述肯尼亚西部艾滋病毒抗体和艾滋病的流行情况，对从603名肯尼亚人滤纸采集的全血进行了包括酶联免疫吸附测定（ELISA）在内的血清学检测，部分样本还进行了免疫印迹检测。其中55人的血清样本也进行了同样检测，45人进一步接受了免疫荧光和一种商用ELISA检测。接受检测的肯尼亚人大多是肯尼亚西部一个省份的居民，其他则是来自肯尼亚其他地区（主要是农村地区）的学生。男女比例为62/38。年龄中位数为18岁（范围0 - 70岁）。对5名先前已证实有艾滋病毒抗体的丹麦人和10名丹麦对照者的滤纸全血及血清进行了艾滋病毒抗体的ELISA和免疫印迹检测。发现对滤纸血进行的检测是可靠的。接受检测的肯尼亚人中只有1人通过ELISA和免疫印迹检测均呈艾滋病毒抗体阳性，患病率为0.17%（95%置信区间：0.00 - 0.93%）。这一低患病率与肯尼亚农村地区先前公布的结果不一致。
