The Key Laboratory of Carbohydrate Chemistry & Biotechnology, Ministry of Education; School of Biotechnology, Jiangnan University, Wuxi 214122, China.
School of Medicine, Jiangnan University, Wuxi 214122, China.
J Agric Food Chem. 2020 Nov 11;68(45):12692-12701. doi: 10.1021/acs.jafc.0c04605. Epub 2020 Nov 2.
Bovine milk-derived exosomes (BMDEs) have potential applications in the pharmaceutical industry as drug delivery carriers. A comprehensive analysis of protein glycosylation in exosomes is necessary to elucidate the process of targeted delivery. In this work, free oligosaccharides (FOSs), -glycans, and -glycans in BMDEs and whey were first analyzed through multiple derivation strategies. In summary, 13 FOSs, 44 -glycans, and 94 -glycans were identified in bovine milk. To analyze site-specific glycosylation of glycoproteins, a one-step method was used to enrich and characterize intact glycopeptides. A total of 1359 proteins including 114 glycoproteins were identified and most of these were located in the exosomes. Approximately 95 glycopeptides were initially discovered and 5 predicted glycosites were confirmed in BMDEs. Collectively, these findings revealed the characterization and distribution of glycans and glycoproteins in BMDEs, providing insight into the potential applications of BMDEs in drug delivery and food science.
牛源细胞外囊泡（BMDEs）作为药物递送载体，在制药行业具有潜在应用。为了阐明靶向递送的过程，需要对囊泡中的蛋白质糖基化进行全面分析。在这项工作中，首先通过多种衍生策略分析了 BMDEs 和乳清中的游离寡糖（FOSs）、β-聚糖和β-聚糖。总之，在牛乳中鉴定出 13 种 FOSs、44 种β-聚糖和 94 种β-聚糖。为了分析糖蛋白的位点特异性糖基化，采用一步法对完整糖肽进行富集和表征。共鉴定出 1359 种蛋白质，包括 114 种糖蛋白，其中大部分位于外囊泡中。最初发现了约 95 种糖肽，并在 BMDEs 中证实了 5 个预测的糖基化位点。综上所述，这些发现揭示了 BMDEs 中聚糖和糖蛋白的特征和分布，为 BMDEs 在药物递送和食品科学中的潜在应用提供了新的思路。
