Department of Chemical, Energy and Environmental Engineering, Faculty of Environmental and Urban Engineering, Kansai University, Japan; Development Headquarters, Milbon Co. Ltd., Japan.
Department of Chemical, Energy and Environmental Engineering, Faculty of Environmental and Urban Engineering, Kansai University, Japan.
Toxicol In Vitro. 2021 Feb;70:105039. doi: 10.1016/j.tiv.2020.105039. Epub 2020 Oct 31.
Concerns regarding animal welfare have led to the need for alternatives to animal eye irritation tests. The reconstructed human cornea-like epithelium (RhCE) test is described in the OECD TG 492 as an alternative to animal eye irritation tests. However, the accuracy and labor investment of this method can be improved if the results can be predicted before the experiment. In this study, we evaluated whether Hansen solubility parameter (HSP) values can be used to predict the results of RhCE method using the LabCyte CORNEA-MODEL for 65 test substances. We found that HSP values can predict the RhCE method with high correlation (accuracy 84.6% (55/65), false-negative rate of 16.2% (7/43), and false-positive rate of 13.6% (3/22). These results indicate that HSP values can be used to predict the results RhCE method using LabCyte CORNEA-MODEL with high reproducibility, and thus are useful for evaluating the safety of substances.
人们对动物福利的关注促使我们需要寻找替代动物眼刺激性试验的方法。OECD TG 492 中将人重建角膜样上皮（RhCE）试验描述为替代动物眼刺激性试验的方法。然而，如果能够在实验前预测实验结果，该方法的准确性和劳动力投入都可以得到改善。在本研究中，我们使用 LabCyte CORNEA-MODEL 评估了 Hansen 溶解度参数（HSP）值是否可以用于预测 65 种测试物质的 RhCE 方法的结果。结果发现，HSP 值可以高度准确地预测 RhCE 方法（准确率为 84.6%（55/65），假阴性率为 16.2%（7/43），假阳性率为 13.6%（3/22））。这些结果表明，HSP 值可以与 LabCyte CORNEA-MODEL 联合使用来预测 RhCE 方法的结果，并且具有高度的重现性，因此可用于评估物质的安全性。
