Singh Mahak, Talimoa Mollier R, Sharma Ph Romen, Kadirvel G, Doley S, Sanjukta R K, Rajkhowa D J, Kandpal B K, Kumar Dinesh, Khan M H, Mitra A
Animal Reproduction Laboratory, ICAR Research Complex for NEH Region, Nagaland Centre, Medziphema, Nagaland, 797106, India.
Animal Reproduction Laboratory, ICAR Research Complex for NEH Region, Nagaland Centre, Medziphema, Nagaland, 797106, India.
Theriogenology. 2021 Jan 1;159:123-131. doi: 10.1016/j.theriogenology.2020.10.023. Epub 2020 Oct 24.
Environmental stress in the form of high temperature humidity index (THI) in tropical and sub-tropical region negatively affects semen quality and fertility of boar. Therefore, the present study was done to evaluate the effect of supplementing flaxseed oil (FLO) to boar's diet on its semen quality, antioxidant status, fatty acid composition of seminal plasma and fertility under sub-tropical climate. For this purpose, six Hampshire crossbreed (50% Hampshire and 50% Gunghroo) boars were divided into two groups i.e control (CON) and treatment (FLO). In FLO and CON group, flaxseed and vegetable oil, respectively, was top dressed at the rate of 3% in basal diets for each boar on daily basis for 16 weeks during monsoon season. A total of 60 ejaculates, comprising 30 ejaculates from each group (ten ejaculates from each boar) were collected. Semen samples were evaluated for sperm quality parameters (SQPs: motility, viability, abnormality, acrosomal integrity and Hypo-osmotic swelling test) and velocity attributes by computer assisted semen analysis (CASA) at fresh and after 72 h of preservation at 17 °C. Antioxidant (glutathione peroxidase; GPx, catalase; CAT, total antioxidant capacity; TAC and malondialdehyde; MDA) were analyzed in seminal plasma and serum. Fatty acid compositions of seminal plasma were analyzed by gas chromatography-mass spectrometry (GC-MS). In-vivo fertility study was also conducted. Reaction time and false mounts were significantly (p < 0.05) reduced in FLO group as compared to CON group. Semen quality parameters were significantly (p < 0.05) improved at fresh stage and after 72 h of liquid storage in FLO group as compared to CON group. Velocity attributes (VAP, VSL, VCL, ALH, BCF and LIN) were significantly (p < 0.05) higher in FLO group. Flaxseed oil supplementation significantly (p < 0.01) enhanced serum GPx and CAT concentration. Serum and seminal plasma MDA concentration decreased significantly (p < 0.01) in FLO group. Similarly, GPx, TAC and CAT were significantly (p < 0.01) elevated in seminal plasma of FLO group. The study revealed that feeding of flaxseed oil altered the fatty acid composition of seminal plasma and significantly (p < 0.05) improved the farrowing rate. In summary, flaxseed oil supplementation improved the semen quality parameters and fertility of boars in sub-tropical climate by improving the antioxidant capacity and altering the fatty acid composition of seminal plasma.
热带和亚热带地区高温湿度指数（THI）形式的环境应激会对公猪的精液质量和繁殖力产生负面影响。因此，本研究旨在评估在亚热带气候条件下，在公猪日粮中添加亚麻籽油（FLO）对其精液质量、抗氧化状态、精浆脂肪酸组成和繁殖力的影响。为此，将六头汉普夏杂交（50%汉普夏和50%贡格鲁）公猪分为两组，即对照组（CON）和处理组（FLO）。在季风季节，FLO组和CON组分别以3%的比例在每头公猪的基础日粮中每日额外添加亚麻籽和植物油，持续16周。共采集了60份精液，每组30份（每头公猪10份）。对精液样本进行精子质量参数（SQPs：活力、存活率、畸形率、顶体完整性和低渗肿胀试验）评估，并通过计算机辅助精液分析（CASA）在新鲜时以及在17°C保存72小时后评估速度属性。分析精浆和血清中的抗氧化剂（谷胱甘肽过氧化物酶；GPx、过氧化氢酶；CAT、总抗氧化能力；TAC和丙二醛；MDA）。通过气相色谱-质谱联用（GC-MS）分析精浆的脂肪酸组成。还进行了体内繁殖力研究。与CON组相比，FLO组的反应时间和假爬跨次数显著（p<0.05）减少。与CON组相比，FLO组在新鲜阶段和液体保存72小时后的精液质量参数显著（p<0.05）改善。FLO组的速度属性（VAP、VSL、VCL、ALH、BCF和LIN）显著（p<0.05）更高。添加亚麻籽油显著（p<0.01）提高了血清GPx和CAT浓度。FLO组的血清和精浆MDA浓度显著（p<0.01）降低。同样，FLO组精浆中的GPx、TAC和CAT显著（p<0.01）升高。研究表明，饲喂亚麻籽油改变了精浆的脂肪酸组成，并显著（p<0.05）提高了产仔率。总之，在亚热带气候条件下，添加亚麻籽油通过提高抗氧化能力和改变精浆脂肪酸组成，改善了公猪的精液质量参数和繁殖力。
