Department of Animal Sciences, Tshwane University of Technology, Private Bag X680, Pretoria, 0001, South Africa.
Agricultural Research Council, Germplasm, Conservation, Reproductive Biotechnologies, Private Bag X02, Irene, 0062, South Africa.
Trop Anim Health Prod. 2024 Jul 10;56(6):200. doi: 10.1007/s11250-024-04057-0.
The purpose of this study was to evaluate how ascorbic acid with dietary flaxseed oil affects the quality and fertility of cryopreserved ram sperm in South African indigenous rams. Treatment diets were supplemented 60 days before semen collection to afford proper spermatogenesis, adaptation to the feed formulated and fed throughout the study. Semen was collected with the use of artificial vagina following dietary supplementation with five treatment diets (neg. cont. - negative control, pos. cont. - positive control, FLO - 5% Flaxseed oil, ASA - 4% Ascorbic acid, and FLO + ASA). Semen was then extended using tris-based extender and cryopreserved using the programmable freezer (CBS Freezer 2100 series, Laboratory consumables & chemical suppliers, America). Ovaries were collected from a neighbouring slaughter house and conveyed to the lab in 0.9% saline at 37 °C. Data (sperm parameters and in vitro fertility) was then exposed to the GLM (General Linear Model) in Minitab 17. Pearson's correlation coefficient was utilized to investigate the relationship between cryopreserved sperm quality and in vitro fertility. The student Least Significant Difference Test was used to separate the treatment means, and differences were accepted when the p-value was less than 0.05. The FLO + ASA group had higher (p < 0.05) progressive (36.33 ± 1.87), total (88.24 ± 2.24), rapid motility (27.52 ± 1.74), intact plasma membrane (75.67 ± 2.08), total fertilization (65.98 ± 7.39), and total cleavage (66.19 ± 6.50) when compared to other treatment groups. Total fertilization rate had a medium significant (p < 0.001) medium correlation with the progressive motility (r2 = 0.435), total motility (r = 0.447) and rapid motility (r = 0.409). In conclusion, dietary flaxseed and ascorbic acid (FLO + ASA) improves cryopreserved semen quality, in vitro fertilization rate, and the total cleavage rate. Noteworthy, the progressive, total and rapid motility play a crucial in the in vitro fertilization rate.
本研究旨在评估添加日粮亚麻籽油和抗坏血酸对南非本土绵羊冷冻精子质量和生育力的影响。在收集精液前 60 天，治疗组日粮进行补充，以提供适当的精子发生、适应整个研究期间使用的配方饲料。使用人工阴道收集精液，同时使用五种处理日粮（负对照-阴性对照、正对照-阳性对照、FLO-5%亚麻籽油、ASA-4%抗坏血酸和 FLO+ASA）进行日粮补充。然后使用基于 tris 的稀释液对精液进行扩展，并使用可编程冷冻机（CBS Freezer 2100 系列，Laboratory consumables & chemical suppliers，America）进行冷冻保存。从附近的屠宰场收集卵巢，并在 37°C 的 0.9%盐水中运送到实验室。然后将数据（精子参数和体外受精）暴露于 Minitab 17 中的 GLM（通用线性模型）。利用 Pearson 相关系数研究冷冻精子质量与体外受精的关系。使用学生最小显著差异检验来分离处理均值，当 p 值小于 0.05 时，接受差异。FLO+ASA 组的精子具有更高的活力（p<0.05），如前向运动（36.33±1.87）、总活力（88.24±2.24）、快速运动（27.52±1.74）、完整质膜（75.67±2.08）、总受精率（65.98±7.39）和总卵裂率（66.19±6.50），与其他处理组相比。总受精率与前向运动（r2=0.435）、总活力（r=0.447）和快速运动（r=0.409）呈中度显著相关（p<0.001）。结论：日粮亚麻籽油和抗坏血酸（FLO+ASA）可提高冷冻精子质量、体外受精率和总卵裂率。值得注意的是，前向运动、总活力和快速运动对体外受精率起着至关重要的作用。
