Kargar Rohollah, Forouzanfar Mohsen, Ghalamkari Gholamreza, Nasr Esfahani Mohammad Hossein
Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
Transgenesis Center of Excellence, Isfahan (Khorasgan) Branch, Islamic Azad University, Isfahan, Iran.
Cryobiology. 2017 Feb;74:110-114. doi: 10.1016/j.cryobiol.2016.11.007. Epub 2016 Nov 25.
Semen cryopreservation is affected by individual differences and use of clones animal from the same source is the main tool to eliminate genetic variation. Among many nutrients that are necessary for fertility, essential fatty acids and antioxidants are vital for production of healthy sperm by improving sperm membrane integrity and protecting sperm from oxidative stress. The goal of the current study was to investigate whether a flax seed oil or/and Vitamin E dietary supplementation could improve semen quality of cloned bucks following semen cryopreservation. Accordingly, eight adult cloned Bakhtiari bucks were divided randomly into four groups. Bucks were offered a base diet of hay and concentrate. The concentrate was enriched with flax seed oil, 30 gr/kg body weight/day (OIL), Vitamin E (VIT), 3 gr/kg body weight/day, or combined flax seed oil and the vitamin E (OIL-VIT). The concentrate with no supplements was considered as control group (CONT). Both flax seed oil and Vitamin E supplements were added to the total diet. The bucks were fed with their corresponding diets for a total of 9 weeks while sperm collection was carried out within 10-14 weeks. Ejaculates were diluted with Andromed and were frozen in liquid nitrogen. Sperm parameters and reactive oxygen species (ROS) contents were evaluated following freezing/thawing. According to the results of our study, dietary supplementation with flax seed oil, or/and Vitamin E can improve sperm motility, vitality and number of sperm with intact plasma membrane following freezing-thawing. But the degree of improvement in these parameters was significantly higher when Flax seed oil and vitamin E were co-supplemented.
精液冷冻保存受个体差异影响，使用同一来源的克隆动物是消除遗传变异的主要手段。在众多生育所需的营养物质中，必需脂肪酸和抗氧化剂对于通过改善精子膜完整性和保护精子免受氧化应激来产生健康精子至关重要。本研究的目的是调查亚麻籽油或/和维生素E饮食补充是否能改善克隆雄鹿精液冷冻保存后的精液质量。因此，将八只成年克隆巴赫蒂亚里雄鹿随机分为四组。给雄鹿提供干草和精饲料的基础日粮。精饲料中添加亚麻籽油，30克/千克体重/天（油组）、维生素E（维生素E组），3克/千克体重/天，或同时添加亚麻籽油和维生素E（油-维生素E组）。不添加任何补充剂的精饲料作为对照组（对照组）。亚麻籽油和维生素E补充剂均添加到总日粮中。给雄鹿喂食相应日粮共9周，同时在10-14周内采集精子。精液用安多美稀释并在液氮中冷冻。冷冻/解冻后评估精子参数和活性氧（ROS）含量。根据我们的研究结果，日粮中补充亚麻籽油或/和维生素E可提高冷冻-解冻后精子的活力、存活率和完整质膜精子数量。但当亚麻籽油和维生素E联合补充时，这些参数的改善程度显著更高。
