State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Department of Water Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
State Key Joint Laboratory of Environmental Simulation and Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; Department of Water Pollution Control, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; School of Chemical & Environmental Engineering, China University of Mining & Technology (Beijing), Beijing 100083, China.
Bioresour Technol. 2021 Jan;320(Pt A):124297. doi: 10.1016/j.biortech.2020.124297. Epub 2020 Oct 22.
This study demonstrated the feasibility of anaerobic ammonia oxidation (anammox) served as tertiary nitrogen removal process. An upflow fixed-bed reactor (UFBR) pre-inoculated with anammox bacteria (AnAOB) followed an anoxic/oxic (A/O) reactor treating magnetic-coagulation pretreated municipal wastewater. When bypassing 15% of influent into UFBR, UFBR removed 5.37 mg-TN/L contributing to 23.4% on total TN removal, in which the combination of partial nitritation and partial denitrification with anammox was main nitrogen removal pathway. Relatively low concentrations of NH-N and anaerobic environment promoted the growth of ammonia oxidizing archaea (AOA) in the inner-layer of biofilm in UFBR. The cooperation of AOA and ammonia-oxidizing bacteria (AOB) with AnAOB was achieved, with AOA, AOB, and AnAOB abundances of 0.01-0.32%, 0.25-0.44%, and 0.77-2.18% on the biofilm, respectively. Metagenomic analysis found that although AOB was the main NH-N oxidizer, archaeal amo gene on biofilm increased threefold during 90 days' treatment.
本研究证明了厌氧氨氧化（anammox）作为三级脱氮工艺的可行性。上流式固定床反应器（UFBR）采用接种厌氧氨氧化菌（AnAOB），随后采用缺氧/好氧（A/O）反应器处理磁混凝预处理后的城市污水。当将 15%的进水绕过 UFBR 时，UFBR 去除了 5.37mg-TN/L，对总 TN 去除的贡献率为 23.4%，其中部分硝化和部分反硝化与厌氧氨氧化的组合是主要的脱氮途径。NH-N 浓度较低和厌氧环境促进了 UFBR 生物膜内层氨氧化古菌（AOA）的生长。AOA、氨氧化菌（AOB）和厌氧氨氧化菌（AnAOB）之间实现了合作，生物膜上的 AOA、AOB 和 AnAOB 的丰度分别为 0.01-0.32%、0.25-0.44%和 0.77-2.18%。宏基因组分析发现，虽然 AOB 是主要的 NH-N 氧化菌，但在 90 天的处理过程中，生物膜上的古菌 amo 基因增加了三倍。
