School of Environmental and Municipal Engineering, Qingdao University of Technology, Huangdao District, Qingdao, 266525, People's Republic of China.
School of Marine Science and Technology, Harbin Institute of Technology, Weihai, 264209, People's Republic of China.
Bioprocess Biosyst Eng. 2023 Jul;46(7):1065-1073. doi: 10.1007/s00449-023-02885-2. Epub 2023 May 22.
The enhancement of nitrogen removal was reinforced by nitritation/anammox in an anaerobic/oxic/anoxic (AOA) system of integrated fixed biofilm activated sludge. Nitritation was first attained by the method of free nitrous acid (FNA) inhibition with ammonia residues, and anaerobic ammonia oxidizing bacteria (AnAOB) were then added into the system, which enabled the occurrence of nitritation coupled with anaerobic ammonia oxidation (anammox). The results indicated that nitrogen removal was enhanced by the nitritation/anammox pathway with an efficiency of 88.9%. A microbial analysis showed that the ammonia oxidizing bacterium (AOB) Nitrosomonas was enriched on the biofilm (5.98%) and in the activated sludge (2.40%), and the AnAOB Candidatus Brocadia was detected on the biofilm with a proportion of 0.27%. Nitritation/anammox was attained and maintained due to the accumulation of functional bacteria.
在厌氧/好氧/缺氧（AOA）集成固定生物膜活性污泥系统中，通过游离亚硝酸（FNA）抑制氨残留的方法首先实现了硝化作用，然后向系统中添加厌氧氨氧化菌（AnAOB），从而使硝化作用与厌氧氨氧化（anammox）偶联发生。结果表明，通过硝化/厌氧氨氧化途径，氮去除效率提高到 88.9%。微生物分析表明，氨氧化菌（AOB）亚硝化单胞菌在生物膜（5.98%）和活性污泥（2.40%）中得到富集，而 AnAOB 候选布罗卡氏菌在生物膜中的检出率为 0.27%。由于功能菌的积累，实现并维持了硝化/厌氧氨氧化。
