College of Life Sciences, Northeast Agricultural University, Harbin, China.
Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Theor Appl Genet. 2021 Feb;134(2):505-518. doi: 10.1007/s00122-020-03710-0. Epub 2020 Nov 2.
Anatomical changes in and hormone roles of the exserted stigma were investigated, and localization and functional analysis of SlLst for the exserted stigma were performed using SLAF-BSA-seq, parental resequencing and overexpression of SlLst in tomato. Tomato accession T431 produces stigmas under relatively high temperatures (> 27 °C, the average temperature in Harbin, China, in June-August), so pollen can rarely reach the stigma properly. This allows the percentage of male sterility exceed 95%, making the use of this accession practical for hybrid seed production. To investigate the mechanism underlying the exserted stigma male sterility, the morphological changes of, anatomical changes of, and comparative endogenous hormone (IAA, ABA, GA, ZT, SA) changes in flowers during flower development of tomato accessions DL5 and T431 were measured. The location and function of genes controlling exserted stigma sterility were analyzed using super SLAF-BSA-seq, parental resequencing, comparative genomics and the overexpression of SlLst in tomato. The results showed that an increase in cell number mainly caused stigma exsertion. IAA played a major role, while ABA had an opposite effect on stigma exertion. Moreover, 26 candidate genes related to the exserted stigma were found, located on chromosome 12. The Solyc12g027610.1 (SlLst) gene was identified as the key candidate gene by functional analysis. A subcellular localization assay revealed that SlLst is targeted to the nucleus and cell membrane. Phenotypic analysis of SlLst-overexpressing tomato showed that SlLst plays a crucial role during stigma exsertion.
对伸出柱头的解剖学变化和激素作用进行了研究，并利用 SLAF-BSA-seq、双亲重测序和在番茄中过表达 SlLst 对伸出柱头的 SlLst 进行了定位和功能分析。番茄品种 T431 在相对较高的温度（>27°C，中国哈尔滨 6-8 月的平均温度）下产生柱头，因此花粉很少能正常到达柱头。这使得雄性不育率超过 95%，使得该品种在杂种种子生产中实际应用成为可能。为了研究伸出柱头雄性不育的机制，测量了番茄品种 DL5 和 T431 花发育过程中花朵的形态变化、解剖结构变化以及比较内源激素（IAA、ABA、GA、ZT、SA）的变化。利用超 SLAF-BSA-seq、双亲重测序、比较基因组学和在番茄中过表达 SlLst 分析了控制伸出柱头不育的基因的位置和功能。结果表明，细胞数量的增加主要导致柱头伸出。IAA 起主要作用，而 ABA 对柱头伸出有相反的作用。此外，在 12 号染色体上发现了 26 个与伸出柱头相关的候选基因。鉴定到 Solyc12g027610.1（SlLst）基因为关键候选基因。亚细胞定位试验表明 SlLst 靶向细胞核和细胞膜。SlLst 过表达番茄的表型分析表明，SlLst 在柱头伸出过程中起着至关重要的作用。
