College of Life Sciences, Northeast Agricultural University, Harbin 150030, People's Republic of China.
Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou 510640, People's Republic of China.
Phytopathology. 2021 Mar;111(3):485-495. doi: 10.1094/PHYTO-06-20-0242-R. Epub 2021 Feb 9.
Chloroplast ATP synthase (cpATPase) is responsible for ATP production during photosynthesis. Our previous studies showed that the cpATPase CF1 α subunit (AtpA) is a key protein involved in -induced resistance to the fungus in tomato. Here, we show that expression of the tomato gene was upregulated by and The tomato gene was then isolated, and transgenic tobacco lines were obtained. Compared with untransformed plants, -overexpressing tobacco showed increased resistance to , characterized by reduced disease incidence, defense-associated hypersensitive response-like reactions, balanced reactive oxygen species, alleviated damage to the chloroplast ultrastructure of leaf cells, elevated levels of ATP content and cpATPase activity, and enhanced expression of genes related to carbon metabolism, photosynthesis, and defense. Incremental Ca efflux and steady H efflux were observed in transgenic tobacco after inoculation with . In addition, overexpression of conferred enhanced tolerance to salinity and resistance to the fungus . Thus, AtpA is a key regulator that links signaling to cellular redox homeostasis, ATP biosynthesis, and gene expression of resistance traits to modulate immunity to pathogen infection and provides broad-spectrum resistance in plants in the process.
叶绿体 ATP 合酶（cpATPase）负责光合作用过程中的 ATP 生成。我们之前的研究表明，cpATPase CF1α 亚基（AtpA）是参与番茄对真菌 - 诱导抗性的关键蛋白。在这里，我们表明 和 可以上调番茄 基因的表达。随后，我们分离了番茄 基因，并获得了转基因烟草株系。与未转化的植物相比，过表达 的烟草对 表现出更高的抗性，其特征为发病程度降低、防御相关的过敏反应样反应、活性氧平衡、叶片细胞叶绿体超微结构损伤减轻、ATP 含量和 cpATPase 活性升高，以及与碳代谢、光合作用和防御相关的基因表达增强。在接种 后，转基因烟草中观察到 Ca 不断外排和 H 持续外排。此外， 的过表达赋予了转基因烟草对盐度的耐受性增强和对真菌的抗性增强。因此，AtpA 是一个关键的调节剂，它将信号传递与细胞氧化还原稳态、ATP 生物合成以及抗性性状的基因表达联系起来，从而调节植物对病原体感染的免疫反应，并在这个过程中提供广谱抗性。
