Cellular and Molecular Research Center, Qom University of Medical Sciences, Qom, Iran.
Department of Tissue Engineering, School of Medicine, Qom University of Medical Sciences, Qom, Iran.
J Mater Sci Mater Med. 2020 Nov 2;31(11):101. doi: 10.1007/s10856-020-06433-2.
To boost the healing process in a full-thickness wound, a simple and efficient strategy based on adipose-derived mesenchymal stem cells (ADSCs) transplantation is described in this work. To increase the chance of ADSCs immobilization in the wound bed and prevent its migration, these cells are fully grown on the surface of a thermoresponsive dressing membrane under in vitro condition. Then, the cells sheet with their secreted extracellular matrix (ECM) is transferred to the damaged skin with the help of this dressing membrane. This membrane remains on wound bed and acts both as a cell sheet transfer vehicle, after external reduction of temperature, and protect wound during the healing process like a common wound dressing. The visual inspection of wounded skin (rat animal model) at selected time intervals shows a higher wound closure rate for ADSCs treated group. For this group of rats, the better quality of reconstructed tissue is approved by results of histological and immunohistochemical analysis since the higher length of the new epidermis, the higher thickness of re-epithelialization layer, a higher level of neovascularization and capillary density, and the least collagen deposition are detected in the healed tissue.
为了促进全层创面的愈合过程，本工作中描述了一种基于脂肪来源间充质干细胞（ADSCs）移植的简单有效的策略。为了增加 ADCS 在创面床中的固定机会并防止其迁移，这些细胞在体外条件下完全生长在热敏性敷料膜的表面上。然后，借助该敷料膜将带有其分泌的细胞外基质（ECM）的细胞片转移到受损的皮肤上。该膜保留在创面床上，在降低外部温度后充当细胞片转移载体，并且像普通的创面敷料一样在愈合过程中保护创面。在选定的时间间隔对受伤皮肤（大鼠动物模型）进行的目视检查显示，接受 ADSC 治疗的组的创面闭合率更高。对于该组大鼠，组织学和免疫组织化学分析的结果证实了重建组织的更好质量，因为在愈合组织中检测到新表皮的长度更长，再上皮化层的厚度更高，新生血管化和毛细血管密度更高，以及胶原沉积最少。
