Department of Medical Nanotechnology, School of Advanced Medical Technologies, Tehran University of Medical Sciences, Tehran, Iran.
Department of Laboratory Sciences, Faculty of Paramedicine, Yasuj University of Medical Sciences, Yasuj, Iran.
Cell Tissue Res. 2019 Mar;375(3):709-721. doi: 10.1007/s00441-018-2927-6. Epub 2018 Oct 18.
Full-thickness skin defect is one of the main clinical problems, which cannot be repaired spontaneously. The aim of this study was to evaluate the feasibility of combining nanofibers with ADM as a bilayer scaffold for treatment of full-thickness skin wounds in a single-step procedure. The nanofibrous polycaprolactone/fibrinogen scaffolds were fabricated by electrospinning. Subsequently, mesenchymal stem cells were isolated from rat adipose tissues and characterized by flow cytometry. Cell adhesion, proliferation, and the epidermal differentiation potential of adipose-derived stem cells (ADSCs) on nanofibrous scaffolds were investigated by scanning electron microscopy (SEM), alamarBlue, and real-time PCR, respectively. In animal studies, full-thickness excisional wounds were created on the back of rats and treated with following groups: ADM, ADM-ADSCs, nanofiber, nanofiber-ADSCs, bilayer, and bilayer-ADSCs. In all groups, wounds were harvested on days 14 and 21 after treatment to evaluate re-epithelialization, blood vessel density, and collagen content. The results indicated that ADSCs seeded on ADM, nanofiber, and bilayer scaffolds can promote re-epithelialization, angiogenesis, and collagen remodeling in comparison with cell-free scaffolds. In conclusion, nanofiber-ADSCs showed the best results for re-epithelialization (according to histological scoring), average blood vessel density (92.7 ± 6.8), and collagen density (87.4 ± 4.9%) when compared to the control and other experimental groups.
全层皮肤缺损是主要的临床问题之一，不能自发修复。本研究旨在评估将纳米纤维与 ADM 结合作为双层支架，一步法治疗全层皮肤创面的可行性。通过静电纺丝制备纳米纤维聚己内酯/纤维蛋白支架。随后，从大鼠脂肪组织中分离间充质干细胞，并通过流式细胞术进行鉴定。通过扫描电子显微镜（SEM）、alamarBlue 和实时 PCR 分别研究脂肪来源干细胞（ADSCs）在纳米纤维支架上的黏附、增殖和表皮分化潜能。在动物研究中，在大鼠背部创建全层切除创面，并以下列组进行治疗：ADM、ADM-ADSCs、纳米纤维、纳米纤维-ADSCs、双层和双层-ADSCs。在所有组中，在治疗后 14 天和 21 天收获创面，以评估再上皮化、血管密度和胶原含量。结果表明，与无细胞支架相比，接种于 ADM、纳米纤维和双层支架上的 ADSCs 可促进再上皮化、血管生成和胶原重塑。总之，与对照组和其他实验组相比，纳米纤维-ADSCs 在再上皮化（根据组织学评分）、平均血管密度（92.7±6.8）和胶原密度（87.4±4.9%）方面表现最佳。
