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Focus issue on best practices for development, validation, and use of PCR assays.

作者信息

Toohey-Kurth Kathy, Crossley Beate, Hietala Sharon

机构信息

California Animal Health and Food Safety Laboratory, University of California-Davis, San Bernardino.

California Animal Health and Food Safety Laboratory, University of California-Davis, Davis branches, CA.

出版信息

J Vet Diagn Invest. 2020 Nov;32(6):757. doi: 10.1177/1040638720967122.

DOI:10.1177/1040638720967122
PMID:33140707
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7649540/
Abstract
摘要

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本文引用的文献

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Best practices for performance of real-time PCR assays in veterinary diagnostic laboratories.兽医诊断实验室实时 PCR 检测分析的最佳操作实践。
J Vet Diagn Invest. 2020 Nov;32(6):815-825. doi: 10.1177/1040638720962076. Epub 2020 Sep 30.
2
Suggested guidelines for validation of real-time PCR assays in veterinary diagnostic laboratories.兽医诊断实验室实时 PCR 检测方法验证的建议指南。
J Vet Diagn Invest. 2020 Nov;32(6):802-814. doi: 10.1177/1040638720960829. Epub 2020 Sep 28.
3
Validation of laboratory tests for infectious diseases in wild mammals: review and recommendations.野生哺乳动物传染病实验室检测的验证:综述与建议。
J Vet Diagn Invest. 2020 Nov;32(6):776-792. doi: 10.1177/1040638720920346. Epub 2020 May 29.
4
Guidelines for Sanger sequencing and molecular assay monitoring.Sanger 测序和分子检测监测指南。
J Vet Diagn Invest. 2020 Nov;32(6):767-775. doi: 10.1177/1040638720905833. Epub 2020 Feb 18.
5
Characterization of a novel real-time PCR assay.一种新型实时 PCR 检测方法的特征描述。
J Vet Diagn Invest. 2020 Nov;32(6):793-801. doi: 10.1177/1040638719890858. Epub 2019 Nov 21.
6
Inhibition monitoring in veterinary molecular testing.兽医分子检测中的抑制物监测。
J Vet Diagn Invest. 2020 Nov;32(6):758-766. doi: 10.1177/1040638719889315. Epub 2019 Nov 18.