Department of Integrative Biomedical Sciences, Institute of Infectious Disease & Molecular Medicine, Faculty of Health Sciences, University of Cape Town, Cape Town 7925, South Africa.
National Research Council Canada, Nanotechnology Research Centre, Biomedical Nanotechnologies, 11421 Saskatchewan Drive NW, Edmonton, Alberta T6G 2M9, Canada.
J Proteome Res. 2021 Jan 1;20(1):94-109. doi: 10.1021/acs.jproteome.0c00650. Epub 2020 Nov 3.
The World Health Organization (WHO) estimates that , the most pathogenic mycobacterium species to humans, has infected up to a quarter of the world's population, with the occurrence of multidrug-resistant strains on the rise. Research into the detailed composition of the cell envelope proteome in mycobacteria over the last 20 years has formed a key part of the efforts to understand host-pathogen interactions and to control the current tuberculosis epidemic. This is due to the great importance of the cell envelope proteome during infection and during the development of antibiotic resistance as well as the search of surface-exposed proteins that could be targeted by therapeutics and vaccines. A variety of experimental approaches and mycobacterial species have been used in proteomic studies thus far. Here we provide for the first time an extensive summary of the different approaches to isolate the mycobacterial cell envelope, highlight some of the limitations of the studies performed thus far, and comment on how the recent advances in membrane proteomics in other fields might be translated into the field of mycobacteria to provide deeper coverage.
世界卫生组织（WHO）估计，对人类最具致病性的分枝杆菌种类已感染了全球四分之一的人口，而且多药耐药菌株的发生率正在上升。在过去的 20 年中，对分枝杆菌细胞包膜蛋白质组的详细组成的研究已成为理解宿主-病原体相互作用和控制当前结核病流行的关键部分。这是由于在感染过程中和抗生素耐药性发展过程中细胞包膜蛋白质组的重要性，以及寻找可能成为治疗药物和疫苗靶点的表面暴露蛋白。迄今为止，已经使用了多种实验方法和分枝杆菌物种进行蛋白质组学研究。在这里，我们首次提供了对分离分枝杆菌细胞包膜的不同方法的广泛总结，强调了迄今为止进行的研究的一些局限性，并评论了其他领域中膜蛋白质组学的最新进展如何转化为分枝杆菌领域，以提供更深入的覆盖范围。
