Szent István University, Kaposvár Campus, Faculty of Agricultural and Environmental Sciences, Institute of Plant Science, Kaposvár, Hungary.
Szent István University, Kaposvár Campus, Faculty of Agricultural and Environmental Sciences, Institute of Nutrition and Product Development Sciences, Kaposvár, Hungary.
PLoS One. 2020 Nov 3;15(11):e0240470. doi: 10.1371/journal.pone.0240470. eCollection 2020.
Biophoton emission is a well-known phenomenon in living organisms, including plant species; however, the underlying mechanisms are not yet well elucidated. Nevertheless, non-invasive stress detection is of high importance when in plant production and plant research. Therefore, the aim of our work was to investigate, whether biophoton emission is suitable for the detection of cadmium stress in the early phase of stress evolution and to identify certain stress-related events that occur rapidly upon cadmium exposure of barley seedlings parallel to biophoton emission measurements. Changes of biophoton emission, chlorophyll content estimation index, ascorbate level, the activity of ascorbate- and guaiacol peroxidase enzymes and lipid oxidation were measured during seven days of cadmium treatment in barley (Hordeum vulgare L.) seedlings. The results indicate that the antioxidant enzyme system responded the most rapidly to the stress caused by cadmium and the lipid oxidation-related emission of photons was detected in cadmium-treated samples as early as one day after cadmium exposure. Furthermore, a concentration-dependent increase in biophoton emission signals indicating an increased rate of antioxidative enzymes and lipid oxidation was also possible to determine. Our work shows evidence that biophoton emission is suitable to identify the initial phase of cadmium stress effectively and non-invasively.
生物发光是生物体中一种众所周知的现象，包括植物物种；然而，其潜在的机制尚未得到很好的阐明。然而，在植物生产和植物研究中，非侵入性的应激检测非常重要。因此，我们的工作旨在研究生物发光是否适合在应激进化的早期阶段检测镉胁迫，并确定在大麦幼苗受到镉暴露的同时平行进行生物发光测量时，发生的某些与应激相关的快速事件。在大麦（Hordeum vulgare L.）幼苗的镉处理的七天期间，测量了生物发光发射、叶绿素含量估算指数、抗坏血酸水平、抗坏血酸和愈创木酚过氧化物酶的活性以及脂质氧化。结果表明，抗氧化酶系统对镉引起的应激反应最为迅速，在镉处理后的第一天就检测到了与脂质氧化相关的光子发射。此外，还可以确定生物发光信号的浓度依赖性增加，表明抗氧化酶和脂质氧化的速率增加。我们的工作表明，生物发光发射适用于有效地、非侵入性地识别镉胁迫的初始阶段。
