National Institute of Crop Science, Tsukuba 305-8518, Japan.
J Proteomics. 2012 Jul 16;75(13):4151-64. doi: 10.1016/j.jprot.2012.05.037. Epub 2012 May 29.
The present study highlights the protective role of β-aminobutyric acid (BABA) in alleviating cadmium (Cd) stress in soybean. Proteomic analyses revealed that out of 66 differentially abundant protein spots in response to Cd challenge, 17 were common in the leaves of BABA-primed and non-primed plants. Oxygen-evolving enhancer protein 1 and ribulose bisphosphate carboxylase small chain 1 were detected in increase abundance in both groups of leaves. Among the 15 commonly decreased protein spots, the relative intensity levels of heat shock cognate 70-kDa protein, carbonic anhydrase, methionine synthase, and glycine dehydrogenase were partially restored after BABA treatment. Moreover, BABA priming significantly enhanced the abundance of the defense-related protein peroxiredoxin and glycolytic enzymes in response to Cd exposure. Additionally, the impact of Cd on the physiological state of BABA-primed and non-primed plants was analyzed using a biophoton technique. The finding of comparatively low biophoton emission in BABA-primed leaves under Cd stress indicates that these plants experienced less oxidative damage than that of non-primed plants. Proteomic study coupled with biophoton analysis reveals that BABA pretreatment helps the plants to combat Cd stress by modulating plants' defence mechanism as well as activating cellular detoxification system to protect the cells from Cd induced oxidative stress damages.
本研究强调了β-氨基丁酸（BABA）在缓解大豆镉（Cd）胁迫中的保护作用。蛋白质组学分析显示，在对 Cd 胁迫反应的 66 个差异丰度蛋白斑点中，有 17 个在 BABA 预处理和未预处理植物的叶片中是共同的。在两组叶片中都检测到氧释放增强蛋白 1 和核酮糖二磷酸羧化酶小链 1 的丰度增加。在 15 个共同减少的蛋白斑点中，热休克同源 70kDa 蛋白、碳酸酐酶、蛋氨酸合酶和甘氨酸脱氢酶的相对强度水平在 BABA 处理后部分恢复。此外，BABA 预处理显著增强了防御相关蛋白过氧化物酶和糖酵解酶在暴露于 Cd 时的丰度。此外，还使用生物光子技术分析了 Cd 对 BABA 预处理和未预处理植物生理状态的影响。在 Cd 胁迫下，BABA 预处理叶片中生物光子发射相对较低的发现表明，这些植物经历的氧化损伤比未预处理植物少。蛋白质组学研究结合生物光子分析表明，BABA 预处理通过调节植物的防御机制以及激活细胞解毒系统来帮助植物对抗 Cd 胁迫，从而保护细胞免受 Cd 诱导的氧化应激损伤。
