Institute of NanoEngineering and MicroSystems, National Tsing Hua University, HsinChu 30013, Taiwan.
Engineering and System Science Dept., National Tsing Hua University, HsinChu 30013, Taiwan.
Biosensors (Basel). 2020 Oct 31;10(11):163. doi: 10.3390/bios10110163.
In this paper, a nanobiosensor with surface-enhanced Raman scattering (SERS) capability is introduced for highly sensitive miRNA detection in colorectal cancer. This sensor was designed and fabricated by employing a nanoshielding mechanism from nanopolystyrene beads to resist reactive ion etching and allow anisotropic electrochemical etching, producing high-aspect-ratio, surface-corrugated nanopillars (SiNPs) on a silicon wafer to create extensive hot spots along the nanopillars for improved SERS signals. SERS enhancements were correlated with nanorange roughness, indicating that hot spots along the pillars were the crucial factor to improve the SERS effect. We achieved the detection capability of a trace amount of R6G (10 M), and the SERS signal enhancement factor (EF) was close to 1.0 × 10 on surface-corrugated gold SiNPs. miRNA samples were also demonstrated on this sensor with good sensitivity and specificity. The target molecule miR-21-Cy5 was easily monitored through Raman spectrum variation with a PCR-comparable concentration at around 100 pM with clear nucleotide-specific Raman signals, which is also suitable for biomolecule sensing.
本文提出了一种具有表面增强拉曼散射（SERS）性能的纳米生物传感器，用于高灵敏度的结直肠癌 miRNA 检测。该传感器采用纳米聚苯乙烯珠的纳米屏蔽机制设计和制造，以抵抗反应离子刻蚀并允许各向异性电化学刻蚀，在硅片上产生高纵横比、表面波纹状纳米柱（SiNPs），沿纳米柱产生广泛的热点，从而提高 SERS 信号。SERS 增强与纳米级粗糙度相关，表明沿支柱的热点是提高 SERS 效应的关键因素。我们实现了对痕量 R6G（10M）的检测能力，表面波纹金 SiNPs 的 SERS 信号增强因子（EF）接近 1.0×10。在该传感器上也展示了 miRNA 样本，具有良好的灵敏度和特异性。目标分子 miR-21-Cy5 通过拉曼光谱随 PCR 可比浓度约为 100pM 的变化很容易被监测，具有清晰的核苷酸特异性拉曼信号，这也适用于生物分子检测。
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