Orosz Enikő, Kiss István, Gyöngyi Zoltán, Varjas Tímea
Institute of National Oncology, Budapest, Hungary.
Department of Public Health Medicine, Medical School, University of Pécs, Pécs, Hungary.
In Vivo. 2018 Nov-Dec;32(6):1333-1337. doi: 10.21873/invivo.11383.
Colorectal cancer is an increasing cause of death. Circulating microRNAs (miRs) could be great diagnostic and prognostic biomarkers of colorectal cancer, but further continuation of their utility is needed for their comprehensive application.
Twenty-seven patients with colonic cancer, 16 with rectal cancer and 12 healthy volunteers as controls, were involved in this study. Expression of miR-155, miR-21, miR-221, miR-30a, miR-34a and miR-29a were determined by reverse transcription polymerase chain reaction (RT-PCR) from sera of patients.
Expression of miR-155, miR-21 and miR-221 was significantly higher in rectal cancer than in colonic cancer. There was no difference found between those with TNM1 cancer and controls for both cancer types. miR-155, miR-34a and miR-29a were down-regulated in all patients with cancer compared to controls. We did not find any statistically significant up-regulation of miR-221 in patients with colonic cancer compared to controls. In contrast, in patients with rectal cancer, miR-221 expression was higher than in controls. Advanced stage was also linked to higher miR-221 expression compared to early stage. Slight, but statistically significant increase was observed in miR-30a expression in patients with colon cancer compared to control individuals.
Our results partly support previous findings. Here we report on differences in the expression of circulating microRNA between colonic and rectal tumours for the first time.
结直肠癌是导致死亡的一个日益重要的原因。循环微小RNA（miRs）可能是结直肠癌很好的诊断和预后生物标志物，但需要进一步延续其效用以实现全面应用。
本研究纳入了27例结肠癌患者、16例直肠癌患者以及12名健康志愿者作为对照。通过逆转录聚合酶链反应（RT-PCR）测定患者血清中miR-155、miR-21、miR-221、miR-30a、miR-34a和miR-29a的表达。
miR-155、miR-21和miR-221在直肠癌中的表达显著高于结肠癌。两种癌症类型中TNM1期癌症患者与对照之间均未发现差异。与对照相比，所有癌症患者的miR-155、miR-34a和miR-29a表达均下调。与对照相比，我们未发现结肠癌患者中miR-221有任何统计学上的显著上调。相反，在直肠癌患者中，miR-221表达高于对照。与早期相比，晚期也与更高的miR-221表达相关。与对照个体相比，结肠癌患者中miR-30a表达有轻微但统计学上显著的增加。
我们的结果部分支持先前的发现。在此我们首次报道了结肠和直肠肿瘤中循环微小RNA表达的差异。
