Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt; Biological Screening and Preclinical Trial Laboratory, Biochemistry Department, Faculty of Science, Alexandria University, Alexandria, Egypt.
Pharmacology and Therapeutics Department, Faculty of Pharmacy, Pharos University in Alexandria, Alexandria, Egypt.
J Chem Neuroanat. 2020 Dec;110:101878. doi: 10.1016/j.jchemneu.2020.101878. Epub 2020 Nov 2.
Alzheimer's disease (AD) is a progressive neurodegenerative disorder with a multifactorial etiology and significantly increasing incidence during the last decade. Hence, developing an effective therapy is crucial for public health. The current study aimed to examine the dual prophylactic/therapeutic potential of a nutraceutical formula based on aqueous extract of roasted date seeds, and nigella and virgin-olive oils against experimentally-induced Alzheimer's disease in rats. Alzheimer's disease-like pathology was induced in male Wistar rats using oral CuSO (200 mg/Kg/day for two months). The nutraceutical formula was given orally to experimental animals (10 mL/kg/d) for 14 days before (as prophylaxis) and after Alzheimer's disease induction and its therapeutic effect in both cases is tested in comparison to donepezil (0.5 mg/kg/d). The nutraceutical formula was found to ameliorate the CuSO-induced neuronal damage and regenerate the affected hippocampus tissue and significantly improvemed in learning ability. The formula was also effective in decreasing brain amyloid-β, tau protein, TNF-α level, iNOS level in hippocampus, oxidative stress level, and inhibiting acetylcholinesterase activity and expression in brain and hippocampus, respectively. Further, an increase in GSH levels, activities of SOD, and GST and levels of hippocampus ADAM 17 and brain phospholipids was observed. In conclusion, the studied nutraceutical formula is proved to be effective in ameliorating Alzheimer's neurodegenerative progression with added-prophylactic potential.
阿尔茨海默病（AD）是一种进行性神经退行性疾病，具有多种病因，在过去十年中发病率显著上升。因此，开发有效的治疗方法对公共健康至关重要。本研究旨在研究基于烤枣仁、黑种草和初榨橄榄油水提物的营养配方对大鼠实验性阿尔茨海默病的双重预防/治疗潜力。使用口服 CuSO（每天 200mg/Kg，持续两个月）在雄性 Wistar 大鼠中诱导类似阿尔茨海默病的病理学。营养配方在阿尔茨海默病诱导前 14 天（作为预防）和之后口服给予实验动物（10mL/kg/d），并测试其在两种情况下的治疗效果，与多奈哌齐（0.5mg/kg/d）进行比较。研究发现，营养配方可改善 CuSO 诱导的神经元损伤，再生受影响的海马组织，并显著改善学习能力。该配方还能有效降低大脑中的淀粉样蛋白-β、tau 蛋白、TNF-α 水平、海马中的 iNOS 水平、氧化应激水平，并分别抑制大脑和海马中的乙酰胆碱酯酶活性和表达。此外，还观察到 GSH 水平、SOD 和 GST 活性以及海马 ADAM 17 和大脑磷脂水平的增加。总之，研究中的营养配方被证明对改善阿尔茨海默病神经退行性进展具有附加预防潜力。
