Department of Anatomy, Embryology and Histology, Faculty of Veterinary Medicine, Assiut University, Assiut, 71526, Egypt.
Department of Pathology, Faculty of Veterinary Medicine, Kafrelsheikh University, Kafr el-Sheikh, 33516, Egypt.
Sci Rep. 2020 Nov 3;10(1):18907. doi: 10.1038/s41598-020-75677-3.
Telocytes comprise the major constituents of the supportive interstitial framework within the various organs. They form a 3D network between different types of stromal and non-stromal cells, which makes them distinctively vital. We have previously explored the origin of the peculiar rodlet cells, especially on their differential stages in aquatic species. The current study aimed at highlighting the relation of telocytes with different rodlet stages. Samples of fish, olfactory organs, and gills were processed for semi thin sections, transmission electron microscopy, and immunohistochemistry. It was evident in the study that telocytes formed a 3D interstitial network, entrapping stem cells and differentiating rodlet cells, to establish direct contact with stem cells. Differentiated stem cells and rodlet progenitor cells, practically in the granular and transitional stages, also formed ultrastructure junctional modifications, by which nanostructures are formed to establish cell contact with telocytes. Telocytes in turn also connected with macrophage progenitor cells. Telocytes (TCs) expressed CD34, CD117, VEGF, and MMP-9. In conclusion, telocytes established direct contact with the stem and rodlet cells in various differential stages. Telocytes may vitally influence stem/progenitor cell differentiation, regulate rodlet cell function, and express MPP-9 that may regulate immune cells functions especially, including movement and migration ability.
间质细胞构成了各种器官内支持性间质框架的主要成分。它们在不同类型的基质细胞和非基质细胞之间形成 3D 网络，这使它们具有独特的重要性。我们之前已经探讨了奇特的杆状细胞的起源，特别是它们在水生物种中的不同分化阶段。本研究旨在强调间质细胞与不同杆状阶段的关系。对鱼类、嗅觉器官和鳃的样本进行半薄切片、透射电子显微镜和免疫组织化学处理。研究表明，间质细胞形成了一个 3D 间质网络，包围着干细胞和分化的杆状细胞，与干细胞建立直接联系。分化的干细胞和杆状祖细胞，实际上处于颗粒和过渡阶段，也形成了超微结构连接的改变，通过这些改变形成纳米结构与间质细胞建立细胞接触。间质细胞反过来也与巨噬细胞祖细胞相连。间质细胞（TCs）表达 CD34、CD117、VEGF 和 MMP-9。总之，间质细胞与不同分化阶段的干细胞和杆状细胞建立了直接联系。间质细胞可能对干细胞/祖细胞的分化具有重要影响，调节杆状细胞的功能，并表达 MMP-9，这可能调节免疫细胞的功能，特别是包括运动和迁移能力。
