Li Min-Jie, Wang Xi, Chen Ying, Li Guang-Jian, Zhao Guang-Qiang, Xiang Bing-Quan, Wei Xue-Qiang, Lei Yu-Jie, Huang Yun-Chao
Department of Thoracic Surgery I, the Third Affiliated Hospital of Kunming Medical University/Yunnan Cancer Hospital, Yunnan Cancer Center, The International Cooperation Key Laboratory of Regional Tumor in High Altitude Area, Kunming, China.
Department of Cardiac Surgery, the Second Affiliated Hospital of Kunming Medical University, Kunming, China.
Ann Transl Med. 2020 Sep;8(17):1076. doi: 10.21037/atm-20-4437.
The cellular immunity of lung cancer patients is mainly the immune response of T cells, which plays an important role in tumour cell killing and immune surveillance. Transforming growth factor 1 (TGF-β1) is secreted by tumour cells that can suppress the immune response and is an important group of immune down-regulation factors. Our study aims to investigate the effect of TGF-β1 on the morphology and cellular immune function of A549 and peripheral blood mononuclear cells (PBMCs).
A549 cell line was cultured, PBMCs were cultured with different concentrations of TGF-β1, and the morphology of A549 cells and PBMCs were seen. The levels of interleukin (IL)-2, IL-4, IL-6, IL-10, IFN-γ, and TNF and the numbers of CD3, CD4, CD8, CD4/CD8, and CD3 CD25 and CD4 CD25 in PBMCs were detected.
During co-culture of A549 with PBMCs, TGF-β1 can induced A549 showing epithelial-to-mesenchymal transition, enhanced its ability of migration and infiltration. Simultaneously, TGF-β1 can depressing the growth and proliferation of PBMCs, inhibiting T-cell activation, and accelerating the PBMCs apoptosis. TGF-β1 can inhibits A549 Th1 related-cytokines, enhance Th2 related-cytokines, cause the disorder of Th1/Th2, resulting in the Th1 cellular dominate immunity decline.
TGF-β1 may affect the secretion of related cytokines, hinder the activation of T lymphocytes, destroy the immune surveillance and killing effect of the body, and thus inhibit the cellular immunity.
肺癌患者的细胞免疫主要是T细胞的免疫反应，在肿瘤细胞杀伤和免疫监视中起重要作用。转化生长因子1（TGF-β1）由肿瘤细胞分泌，可抑制免疫反应，是重要的免疫下调因子组。本研究旨在探讨TGF-β1对A549细胞及外周血单个核细胞（PBMCs）形态和细胞免疫功能的影响。
培养A549细胞系，用不同浓度的TGF-β1培养PBMCs，观察A549细胞和PBMCs的形态。检测PBMCs中白细胞介素（IL）-2、IL-4、IL-6、IL-10、干扰素-γ（IFN-γ）和肿瘤坏死因子（TNF）的水平以及CD3、CD4、CD8、CD4/CD8、CD3 CD25和CD4 CD25的数量。
A549与PBMCs共培养时，TGF-β1可诱导A549发生上皮-间质转化，增强其迁移和浸润能力。同时，TGF-β1可抑制PBMCs的生长和增殖，抑制T细胞活化，并加速PBMCs凋亡。TGF-β1可抑制A549细胞中Th1相关细胞因子，增强Th2相关细胞因子，导致Th1/Th2失衡，致使以Th1细胞为主导的免疫功能下降。
TGF-β1可能影响相关细胞因子的分泌，阻碍T淋巴细胞的活化，破坏机体的免疫监视和杀伤作用，从而抑制细胞免疫。
