Bazzan Erica, Radu Claudia Maria, Tinè Mariaenrica, Neri Tommaso, Biondini Davide, Semenzato Umberto, Casara Alvise, Balestro Elisabetta, Simioni Paolo, Celi Alessandro, Cosio Manuel G, Saetta Marina
Department of Cardiac, Thoracic, Vascular Sciences, and Public Health, University of Padova, Padua, Italy.
Department of Women's and Children's Health, University of Padova, Padua, Italy.
Am J Physiol Lung Cell Mol Physiol. 2021 Feb 1;320(2):L241-L245. doi: 10.1152/ajplung.00362.2020. Epub 2020 Nov 4.
Microvesicles (MVs) released from almost all cells are recognized as cell communication tools. MVs have been investigated in several inflammatory diseases but poorly in biological fluids like bronchoalveolar lavage (BAL) of smokers. The purpose of this study was to investigate the presence and source of MVs in BAL of smokers with and without chronic obstructive pulmonary disease (COPD) compared with nonsmoking controls. Using flow cytometry in BAL, we detected endothelial and alveolar macrophage (AM)-derived MVs and found a higher number of AM-MVs in the BAL of smokers with COPD than in smokers without COPD and nonsmokers, which correlated with the pack-years ( = 0.46; = 0.05) and with the degree of airway obstruction measured by the forced expiratory volume in 1 s percent predicted ( = -0.56; = 0.01). Endothelial and alveolar macrophage-derived MVs are present and measurable in human BAL fluid. In response to smoking and to the development of COPD, inflammatory signals in AM-derived MVs can be quantified, and their numbers are related to the pack-years and the decrease in lung function. These results open the opportunity for future investigation of these microvesicles as biomarkers and possible mechanistic guides in COPD.
几乎所有细胞释放的微泡（MVs）都被视为细胞通讯工具。MVs已在多种炎症性疾病中得到研究，但在吸烟者支气管肺泡灌洗（BAL）等生物流体中的研究较少。本研究的目的是调查患有和未患有慢性阻塞性肺疾病（COPD）的吸烟者BAL中MVs的存在情况和来源，并与非吸烟对照组进行比较。通过对BAL进行流式细胞术检测，我们检测到了内皮细胞和肺泡巨噬细胞（AM）来源的MVs，并发现患有COPD的吸烟者BAL中AM-MVs的数量高于未患有COPD的吸烟者和非吸烟者，这与吸烟包年数相关（r = 0.46；P = 0.05），也与通过1秒用力呼气量占预计值百分比测量的气道阻塞程度相关（r = -0.56；P = 0.01）。内皮细胞和肺泡巨噬细胞来源的MVs存在于人体BAL液中且可测量。针对吸烟和COPD的发展，AM来源的MVs中的炎症信号可以被量化，并且它们的数量与吸烟包年数以及肺功能下降有关。这些结果为未来将这些微泡作为COPD生物标志物和可能的机制指导进行研究提供了机会。
