头孢噻肟在体外微生物学检测期间的稳定性。
Cefotaxime stability during in vitro microbiological testing.
作者信息
Marchbanks C R, Yost R L, White R L
机构信息
College of Pharmacy, Medical University of South Carolina, Charleston 29425.
出版信息
Antimicrob Agents Chemother. 1987 Sep;31(9):1375-8. doi: 10.1128/AAC.31.9.1375.
Cefotaxime is a broad-spectrum cephalosporin which is metabolized or degraded to less active or inactive metabolites by serum esterases, elevated temperatures, or a pH outside of its stability range. Cefotaxime instability during in vitro microbiological susceptibility tests may lead to an underestimation of the antibacterial activity of the compound. Cefotaxime and desacetylcefotaxime solutions were studied under MIC and serum inhibitory titer testing conditions. Cefotaxime concentrations, as measured by high-performance liquid chromatography, decreased 20 to 30% over the incubation period in various systems tested; the greatest decline occurred in systems containing serum in the media. Changes in the results of microbiological susceptibility tests interpreted after 6 and 18 h of incubation were consistent with changes observed in the high-performance liquid chromatography analysis. This study demonstrates cefotaxime instability under conditions of in vitro microbiological testing.
头孢噻肟是一种广谱头孢菌素，它会被血清酯酶、升高的温度或超出其稳定范围的pH值代谢或降解为活性较低或无活性的代谢产物。在体外微生物药敏试验期间，头孢噻肟的不稳定性可能导致对该化合物抗菌活性的低估。在最低抑菌浓度（MIC）和血清抑菌效价测试条件下，对头孢噻肟和去乙酰头孢噻肟溶液进行了研究。通过高效液相色谱法测定，在各种测试系统中，头孢噻肟浓度在孵育期间下降了20%至30%；在培养基中含有血清的系统中下降最为明显。孵育6小时和18小时后解读的微生物药敏试验结果变化与高效液相色谱分析中观察到的变化一致。本研究证明了在体外微生物检测条件下头孢噻肟的不稳定性。
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