Structural Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA.
Structural Biology Program, Sloan Kettering Institute, Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, NY 10065, USA; Howard Hughes Medical Institute, 1275 York Avenue, New York, NY 10065, USA.
Curr Opin Struct Biol. 2021 Apr;67:86-94. doi: 10.1016/j.sbi.2020.09.010. Epub 2020 Nov 2.
The RNA exosome is a conserved complex of proteins that mediates 3'-5' RNA processing and decay. Its functions range from processing of non-coding RNAs such as ribosomal RNAs and decay of aberrant transcripts in the nucleus to cytoplasmic mRNA turnover and quality control. Ski2-like RNA helicases translocate substrates to exosome-associated ribonucleases and interact with the RNA exosome either directly or as part of multi-subunit helicase-containing complexes that identify and target RNA substrates for decay. Recent structures of these helicases with their RNA-binding partners or the RNA exosome have advanced our understanding of a system of modular and mutually exclusive contacts between the exosome and exosome-associated helicase complexes that shape the transcriptome by orchestrating exosome-dependent 3'-5' decay.
RNA 外切体是一种保守的蛋白质复合物,介导 3'-5'RNA 加工和降解。其功能范围从加工非编码 RNA(如核糖体 RNA)和核内异常转录本的降解,到细胞质 mRNA 周转和质量控制。Ski2 样 RNA 解旋酶将底物转运到与外切体相关的核糖核酸酶,并与 RNA 外切体直接或作为多亚基解旋酶复合物的一部分相互作用,该复合物可识别和靶向 RNA 底物进行降解。这些解旋酶与其 RNA 结合伙伴或 RNA 外切体的最近结构,加深了我们对一种模块化和相互排斥的外切体和外切体相关解旋酶复合物之间的系统的理解,该系统通过协调外切体依赖的 3'-5'降解来调控转录组。