• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

破骨细胞前体细胞分泌的 GDNF 诱导骨髓间充质干细胞迁移，并刺激成骨作用。

GDNF secreted by pre-osteoclasts induces migration of bone marrow mesenchymal stem cells and stimulates osteogenesis.

机构信息

Department of Life Science, Ewha Womans University, Seoul 03760; The Research Center for Cellular Homeostasis, Ewha Womans University, Seoul 03760, Korea.

出版信息

BMB Rep. 2020 Dec;53(12):646-651. doi: 10.5483/BMBRep.2020.53.12.199.

DOI:10.5483/BMBRep.2020.53.12.199
PMID:33148376
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC7781916/
Abstract

Bone resorption is linked to bone formation via temporal and spatial coupling within the remodeling cycle. Several lines of evidence point to the critical role of coupling factors derived from pre-osteoclasts (POCs) during the regulation of bone marrowderived mesenchymal stem cells (BMMSCs). However, the role of glial cell-derived neurotrophic factor (GDNF) in BMMSCs is not completely understood. Herein, we demonstrate the role of POC-derived GDNF in regulating the migration and osteogenic differentiation of BMMSCs. RNA sequencing revealed GDNF upregulation in POCs compared with monocytes/macrophages. Specifically, BMMSC migration was inhibited by a neutralizing antibody against GDNF in pre-osteoclast-conditioned medium (POC-CM), whereas treatment with a recombinant GDNF enhanced migration and osteogenic differentiation. In addition, POC-CM derived from GDNF knock-downed bone marrow macrophages suppressed BMMSC migration and osteogenic differentiation. SPP86, a small molecule inhibitor, inhibits BMMSC migration and osteogenic differentiation by targeting the receptor tyrosine kinase RET, which is recruited by GDNF into the GFRα1 complex. Overall, this study highlights the role of POC-derived GDNF in BMMSC migration and osteogenic differentiation, suggesting that GDNF regulates bone metabolism. [BMB Reports 2020; 53(12): 646-651].

摘要

骨吸收通过重塑周期内的时间和空间偶联与骨形成相关联。有几条证据表明，来自破骨前体细胞（POC）的偶联因子在调节骨髓间充质干细胞（BMMSCs）中起着关键作用。然而，胶质细胞衍生的神经营养因子（GDNF）在 BMMSCs 中的作用尚不完全清楚。本文中，我们展示了 POC 衍生的 GDNF 在调节 BMMSCs 迁移和成骨分化中的作用。RNA 测序显示，与单核细胞/巨噬细胞相比，POC 中的 GDNF 上调。具体而言，在破骨前体细胞条件培养基（POC-CM）中，针对 GDNF 的中和抗体抑制了 BMMSC 的迁移，而重组 GDNF 的处理则增强了迁移和成骨分化。此外，源自 GDNF 敲低的骨髓巨噬细胞的 POC-CM 抑制了 BMMSC 的迁移和成骨分化。小分子抑制剂 SPP86 通过靶向 GDNF 招募到 GFRα1 复合物中的受体酪氨酸激酶 RET，从而抑制 BMMSC 的迁移和成骨分化。总的来说，这项研究强调了 POC 衍生的 GDNF 在 BMMSC 迁移和成骨分化中的作用，表明 GDNF 调节骨代谢。[BMB 报告 2020；53(12): 646-651]。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7d5/7781916/d656f880d716/BMB-53-646-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7d5/7781916/1ffbf755fcd2/BMB-53-646-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7d5/7781916/24c25e3b94bf/BMB-53-646-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7d5/7781916/0fbb5de0989a/BMB-53-646-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7d5/7781916/d656f880d716/BMB-53-646-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7d5/7781916/1ffbf755fcd2/BMB-53-646-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7d5/7781916/24c25e3b94bf/BMB-53-646-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7d5/7781916/0fbb5de0989a/BMB-53-646-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/a7d5/7781916/d656f880d716/BMB-53-646-f4.jpg

相似文献

1
GDNF secreted by pre-osteoclasts induces migration of bone marrow mesenchymal stem cells and stimulates osteogenesis.破骨细胞前体细胞分泌的 GDNF 诱导骨髓间充质干细胞迁移，并刺激成骨作用。
BMB Rep. 2020 Dec;53(12):646-651. doi: 10.5483/BMBRep.2020.53.12.199.
2
Non-resorbing osteoclasts induce migration and osteogenic differentiation of mesenchymal stem cells.不可吸收的破骨细胞诱导间充质干细胞的迁移和成骨分化。
J Cell Biochem. 2010 Feb 1;109(2):347-55. doi: 10.1002/jcb.22406.
3
GDNF promotes the proliferation and osteogenic differentiation of jaw bone marrow mesenchymal stem cells via the Nr4a1/PI3K/Akt pathway.GDNF 通过 Nr4a1/PI3K/Akt 通路促进颌骨髓间充质干细胞的增殖和成骨分化。
Cell Signal. 2023 Aug;108:110721. doi: 10.1016/j.cellsig.2023.110721. Epub 2023 May 23.
4
Long non-coding RNA BDNF-AS modulates osteogenic differentiation of bone marrow-derived mesenchymal stem cells.长非编码 RNA BDNF-AS 调节骨髓间充质干细胞的成骨分化。
Mol Cell Biochem. 2018 Aug;445(1-2):59-65. doi: 10.1007/s11010-017-3251-2. Epub 2017 Dec 15.
5
Melatonin up-regulates bone marrow mesenchymal stem cells osteogenic action but suppresses their mediated osteoclastogenesis via MT -inactivated NF-κB pathway.褪黑素通过 MT 失活 NF-κB 通路上调骨髓间充质干细胞成骨作用，但抑制其介导的破骨细胞生成。
Br J Pharmacol. 2020 May;177(9):2106-2122. doi: 10.1111/bph.14972. Epub 2020 Feb 12.
6
Regulation of bone formation by osteoclasts involves Wnt/BMP signaling and the chemokine sphingosine-1-phosphate.破骨细胞对骨形成的调节涉及Wnt/骨形态发生蛋白信号通路和趋化因子鞘氨醇-1-磷酸。
Proc Natl Acad Sci U S A. 2008 Dec 30;105(52):20764-9. doi: 10.1073/pnas.0805133106. Epub 2008 Dec 15.
7
Osteoclast-derived apoptotic bodies show extended biological effects of parental cell in promoting bone defect healing.破骨细胞来源的凋亡小体在促进骨缺损愈合方面表现出亲本细胞的延长的生物学效应。
Theranostics. 2020 May 22;10(15):6825-6838. doi: 10.7150/thno.45170. eCollection 2020.
8
Ca 1.2 regulates osteogenesis of bone marrow-derived mesenchymal stem cells via canonical Wnt pathway in age-related osteoporosis.钙 1.2 通过经典 Wnt 通路调节骨髓间充质干细胞成骨作用在与年龄相关的骨质疏松症中。
Aging Cell. 2019 Aug;18(4):e12967. doi: 10.1111/acel.12967. Epub 2019 May 23.
9
Role of Osteoprotegerin (OPG) in Bone Marrow Adipogenesis.骨保护素（OPG）在骨髓脂肪生成中的作用。
Cell Physiol Biochem. 2016;40(3-4):681-692. doi: 10.1159/000452580. Epub 2016 Nov 30.
10
Osteocytes control myeloid cell proliferation and differentiation through Gsα-dependent and -independent mechanisms.成骨细胞通过 Gsα 依赖和非依赖机制控制髓系细胞的增殖和分化。
FASEB J. 2020 Aug;34(8):10191-10211. doi: 10.1096/fj.202000366R. Epub 2020 Jun 18.

引用本文的文献

1
A Procedural Overview of the Involvement of Small Molecules in the Nervous System in the Regulation of Bone Healing.小分子参与神经系统对骨愈合调节的过程概述
Int J Nanomedicine. 2025 Jan 31;20:1263-1284. doi: 10.2147/IJN.S505677. eCollection 2025.
2
Expression of Glial-Cell-Line-Derived Neurotrophic Factor Family Ligands in Human Intervertebral Discs.胶质细胞源性神经营养因子家族配体在人椎间盘的表达。
Int J Mol Sci. 2023 Nov 1;24(21):15874. doi: 10.3390/ijms242115874.
3
Assembling the Puzzle Pieces. Insights for in Vitro Bone Remodeling.

本文引用的文献

1
GGA3-mediated recycling of the RET receptor tyrosine kinase contributes to cell migration and invasion.GGA3 介导的 RET 受体酪氨酸激酶循环有助于细胞迁移和侵袭。
Oncogene. 2020 Feb;39(6):1361-1377. doi: 10.1038/s41388-019-1068-z. Epub 2019 Oct 23.
2
RACK1 interaction with c-Src is essential for osteoclast function.RACK1与c-Src的相互作用对破骨细胞功能至关重要。
Exp Mol Med. 2019 Jul 29;51(7):1-9. doi: 10.1038/s12276-019-0285-4.
3
The IRF2BP2-KLF2 axis regulates osteoclast and osteoblast differentiation.IRF2BP2-KLF2 轴调节破骨细胞和成骨细胞分化。
拼凑拼图碎片。体外骨重塑的见解。
Stem Cell Rev Rep. 2023 Aug;19(6):1635-1658. doi: 10.1007/s12015-023-10558-6. Epub 2023 May 19.
4
Activation of GDNF-ERK-Runx1 signaling contributes to P2X3R gene transcription and bone cancer pain.胶质细胞源性神经营养因子-细胞外信号调节激酶- Runt相关转录因子1信号通路的激活促进P2X3R基因转录及骨癌痛。
iScience. 2022 Aug 13;25(9):104936. doi: 10.1016/j.isci.2022.104936. eCollection 2022 Sep 16.
5
Fracture Risk in Pediatric Patients With MEN2B.2B 型多发性内分泌瘤病患儿的骨折风险。
J Clin Endocrinol Metab. 2022 Nov 25;107(12):e4371-e4378. doi: 10.1210/clinem/dgac500.
6
Nervous System-Driven Osseointegration.神经系统驱动的骨整合。
Int J Mol Sci. 2022 Aug 10;23(16):8893. doi: 10.3390/ijms23168893.
7
[Research progress of Schwann cells regulating bone regeneration].[雪旺细胞调控骨再生的研究进展]
Zhongguo Xiu Fu Chong Jian Wai Ke Za Zhi. 2022 Feb 15;36(2):236-241. doi: 10.7507/1002-1892.202108153.
8
Genetic Determinants of Inherited Endocrine Tumors: Do They Have a Direct Role in Bone Metabolism Regulation and Osteoporosis?遗传性内分泌肿瘤的遗传决定因素：它们在骨代谢调节和骨质疏松症中是否具有直接作用？
Genes (Basel). 2021 Aug 23;12(8):1286. doi: 10.3390/genes12081286.
BMB Rep. 2019 Jul;52(7):469-474. doi: 10.5483/BMBRep.2019.52.7.104.
4
Mesenchymal Stem Cell Migration during Bone Formation and Bone Diseases Therapy.间质干细胞在骨形成和骨疾病治疗中的迁移。
Int J Mol Sci. 2018 Aug 9;19(8):2343. doi: 10.3390/ijms19082343.
5
Paracrine and endocrine actions of bone-the functions of secretory proteins from osteoblasts, osteocytes, and osteoclasts.骨的旁分泌和内分泌作用——成骨细胞、骨细胞和破骨细胞分泌蛋白的功能
Bone Res. 2018 May 24;6:16. doi: 10.1038/s41413-018-0019-6. eCollection 2018.
6
Human amnion-derived mesenchymal stem cells induced osteogenesis and angiogenesis in human adipose-derived stem cells via ERK1/2 MAPK signaling pathway.人羊膜间充质干细胞通过 ERK1/2 MAPK 信号通路诱导人脂肪来源干细胞成骨和成血管。
BMB Rep. 2018 Apr;51(4):194-199. doi: 10.5483/bmbrep.2018.51.4.005.
7
Glial cell-derived neurotrophic factor promotes dental pulp stem cell migration.胶质细胞源性神经营养因子促进牙髓干细胞迁移。
J Tissue Eng Regen Med. 2018 Mar;12(3):705-714. doi: 10.1002/term.2490. Epub 2017 Oct 19.
8
RAS signalling through PI3-Kinase controls cell migration via modulation of Reelin expression.通过PI3激酶的RAS信号传导通过调节Reelin表达来控制细胞迁移。
Nat Commun. 2016 Apr 13;7:11245. doi: 10.1038/ncomms11245.
9
Targeting the Gdnf Gene in peritubular myoid cells disrupts undifferentiated spermatogonial cell development.靶向管周肌样细胞中的Gdnf基因会破坏未分化精原细胞的发育。
Proc Natl Acad Sci U S A. 2016 Feb 16;113(7):1829-34. doi: 10.1073/pnas.1517994113. Epub 2016 Feb 1.
10
Selective inhibition of RET mediated cell proliferation in vitro by the kinase inhibitor SPP86.激酶抑制剂SPP86在体外对RET介导的细胞增殖的选择性抑制作用。
BMC Cancer. 2014 Nov 20;14:853. doi: 10.1186/1471-2407-14-853.