Virología Clínica y Zoonosis, Hospital Universitario Reina Sofía de Córdoba, Instituto Maimónides de Investigación Biomédica de Córdoba (IMIBIC), Universidad de Córdoba, Córdoba, Spain.
Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Córdoba, Córdoba, Spain.
J Clin Microbiol. 2021 Jan 21;59(2). doi: 10.1128/JCM.02075-20.
The objective of this study was to design a pangenotypic PCR-based assay for the detection and quantification of hepatitis E virus (HEV) RNA from across the entire spectrum of described genotypes belonging to the A genus. The optimal conditions and the performance of the assay were determined by testing the WHO standard strain (6219/10) and the WHO HEV panel (8578/13). Similarly, performance comparisons were made with two commercial assays (Real Star HEV RT-PCR 2.0 and Cube HEV 2.0 Quant) to detect HEV RNA at concentrations below 1,000 IU/ml with viral strains from the WHO and to test samples from 54 patients with acute hepatitis. The assay presented in this study was able to detect the entire spectrum of described genotypes belonging to the A genus, demonstrating better performance than both commercial kits. This procedure may represent a significant improvement in the molecular diagnosis of HEV infection.
本研究旨在设计一种基于泛基因型 PCR 的检测方法，用于检测和定量描述属 A 种的整个基因型的戊型肝炎病毒 (HEV) RNA。通过测试世界卫生组织（WHO）标准株（6219/10）和 WHO HEV 面板（8578/13），确定了最佳条件和检测方法的性能。同样，与两种商业检测试剂盒（Real Star HEV RT-PCR 2.0 和 Cube HEV 2.0 Quant）进行了性能比较，以检测浓度低于 1000 IU/ml 的 WHO 病毒株和 54 例急性肝炎患者样本中的 HEV RNA。本研究中提出的检测方法能够检测到描述属 A 种的整个基因型，其性能优于两种商业试剂盒。该程序可能是戊型肝炎病毒感染分子诊断的重大改进。
